Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer
Joint Authors
Zeng, Chunyan
Zhu, Yin
Zhou, Xiaodong
Hu, Yi
Lu, Nong-Hua
Cheng, Ming
Yang, Zhen
Chen, Jiang
Source
Gastroenterology Research and Practice
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A mouse gastric cancer model is an important tool for studying the mechanisms of gastric cancer.
To establish subcutaneously implanted tumors, MKN-45 cell suspensions and tumor tissues were implanted into the middle of the right armpit of nude mice.
To generate an abdominal metastasis model, MKN-45 cell suspensions and tumor tissue homogenates were implanted into the middle of the lower abdomen.
We measured the weights of the nude mice and the longest dimension, shortest dimension, thickness, and volume of the tumor.
We also analyzed the rate of tumor formation, the time required for tumor formation, and the number and size of abdominal tumors in the mice.
The rates of formation of the subcutaneously implanted tumors were 100%, 0%, and 100% in the nude mice inoculated with 2 × 107 cells/mL or 1 × 107 cells/mL of the MKN-45 cell suspension or the tumor tissue homogenate (2 × 107 cells/mL), respectively.
The rates of metastatic abdominal tumor formation were 100%, 50%, and 75% in mice inoculated with 5 × 107 cells/mL or 1 × 107 cells/mL of the tumor tissue homogenate or the MKN-45 cell suspension (5 × 107 cells/mL), respectively.
We derived tumor tissues and tumor tissue homogenates from nude mice prior to establishing the subcutaneous model of implanted tumors and the abdominal metastasis model of gastric cancer, respectively.
American Psychological Association (APA)
Zhu, Yin& Hu, Yi& Cheng, Ming& Zeng, Chunyan& Yang, Zhen& Zhou, Xiaodong…[et al.]. 2017. Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer. Gastroenterology Research and Practice،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1156556
Modern Language Association (MLA)
Zhu, Yin…[et al.]. Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer. Gastroenterology Research and Practice No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1156556
American Medical Association (AMA)
Zhu, Yin& Hu, Yi& Cheng, Ming& Zeng, Chunyan& Yang, Zhen& Zhou, Xiaodong…[et al.]. Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer. Gastroenterology Research and Practice. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1156556
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156556