3,5-Dicaffeoyl-Epi-Quinic Acid Isolated from Edible Halophyte Atriplex gmelinii Inhibits Adipogenesis via AMPKMAPK Pathway in 3T3-L1 Adipocytes

Abstract EN

Atriplex gmelinii is an edible halophyte that has been suggested to possess various health benefits.

In the present study, 3,5-dicaffeoyl-epi-quinic acid (DEQA) isolated from A.

gmelinii was tested for its ability to prevent adipogenesis in 3T3-L1 cells.

Also, the molecular mechanisms by which DEQA affects differentiation of 3T3-L1 cells were investigated.

The introduction of DEQA to differentiating 3T3-L1 preadipocytes resulted in suppressed adipogenesis and lowered expression of adipogenesis-related factors, PPARγ, C/EBPα, and SREBP-1c.

Treatment of 3T3-L1 adipocytes with DEQA notably decreased the levels of phosphorylated p38, ERK, and JNK.

In addition, presence of DEQA upregulated the levels of both inactive and phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and its substrate, acetyl-CoA carboxylase (ACC).

Taken together, current results indicated that DEQA exhibited a significant antiadipogenesis activity by activation of AMPK and downregulation of MAPK signal pathways in 3T3-L1 preadipocytes.