The Possible Anti-Inflammatory Effect of Dehydrocostus Lactone on DSS-Induced Colitis in Mice
Joint Authors
Chen, Tuo
He, Zongqi
Zhou, Qing
Wu, Bensheng
Chen, Yugen
Shen, Zhao-Feng
Shang, Hong-tao
Han, Shu-tang
Zhang, Wei-xin
Wang, Qiong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Dehydrocostus lactone (DL), one of the main active constituents in Aucklandia lappa Decne.
(Muxiang), reported to have anti-inflammatory, antiulcer, and immunomodulatory properties.
However, the effect of DL on ulcerative colitis (UC) has not been reported.
To analyze the anti-inflammatory potential role of DL in UC, we provide a mechanism for the pharmacological action of DL.
Methods.
The experimental model of UC was induced by using oral administration of 2% dextran sulfate sodium (DSS) with drinking water in BALB/c mice.
Mesalazine (Mes, 0.52 g/kg/d), DL-high doses (DL-H, 20 mg/kg/d), DL-middle doses (DL-M, 15 mg/kg/d), DL-low doses (DL-L, 10 mg/kg/d) were gavaged once a day from day 4 to day 17.
Disease activity index (DAI) was calculated daily.
On day 18, mice were rapidly dissected and the colorectal tissues were used to detect the levels of UC-related inflammatory cytokines (TNF-α, IL-1β, MCP-1, MPO, SOD, IL-6, IL-17, and IL-23), IL-6/STAT3 inflammatory signaling pathway (iNOS, COX2, IL-6, GP130, L-17, and IL-23), and colorectal mucosal barrier-related regulatory factors (MUC2, XBP1s, and α-defensins) by ELISA or qRT-PCR.
Results.
DL reduced the colorectal inflammation histological assessment, decreased UC-related inflammatory cytokines (TNF-α, IL-1β, MCP-1, MPO, SOD, IL-6, IL-17, and IL-23), downregulated IL-6/STAT3 inflammatory signaling pathway (iNOS, COX2, IL-6, GP130, L-17, and IL-23), repaired the key colorectal mucosal barrier protein-MUC2, and inhibited the downstream pathway (XBP1s and α-defensin).
Conclusions.
DL possessed the potential of anti-inflammatory effect to treated colitis.
The protective mechanism of DL may involve in reducing inflammation and improving colorectal barrier function via downregulating the IL-6/STAT3 signaling.
American Psychological Association (APA)
Zhou, Qing& Zhang, Wei-xin& He, Zongqi& Wu, Bensheng& Shen, Zhao-Feng& Shang, Hong-tao…[et al.]. 2020. The Possible Anti-Inflammatory Effect of Dehydrocostus Lactone on DSS-Induced Colitis in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1156642
Modern Language Association (MLA)
Zhou, Qing…[et al.]. The Possible Anti-Inflammatory Effect of Dehydrocostus Lactone on DSS-Induced Colitis in Mice. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1156642
American Medical Association (AMA)
Zhou, Qing& Zhang, Wei-xin& He, Zongqi& Wu, Bensheng& Shen, Zhao-Feng& Shang, Hong-tao…[et al.]. The Possible Anti-Inflammatory Effect of Dehydrocostus Lactone on DSS-Induced Colitis in Mice. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1156642
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156642