Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells HypoxiaReoxygenation Injury via Effects on miR-21 Expression, PTENAkt, and the Bcl-2Bax Pathway

Abstract EN

This study aimed to investigate whether the total flavonoids (TFs) from Carya cathayensis Sarg.

leaves alleviate hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes and to explore potential mechanisms.

H9c2 cells pretreated with TFs for 24h were exposed to H/R treatment.

The results indicated that TFs significantly alleviate H/R injury, which include inhibiting apoptosis and enhancing antioxidant capacity.

The protective effects of TFs resulted in higher expression of miR-21 in H/R-induced H9c2 cells than that of controls, which in turn upregulated Akt signaling activity via suppressing the expression of PTEN together with decreasing the ratio of Bax/Bcl-2, caspase3, and cleaved-caspase3 expression in H/R-induced H9c2 cells.

Conversely, blocking miR-21 expression with miR-21 inhibitor effectively suppressed the protective effects of TFs against H/R-induced injury.

Our study suggests that TFs can decrease cell apoptosis, which may be mediated by altering the expression of miR-21, PTEN/Akt, and Bcl/Bax.