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Biological Evaluation of Different Extracts of Aerial Parts of Nepeta deflersiana and Standardization of Active Extracts Using 8-Epi-7-Deoxyloganic Acid and Ursolic Acid by Validated HPTLC Method
Joint Authors
Khan, Shabana
Perveen, Shagufta
Al-Taweel, Areej Mohammad
Siddiqui, Nasir Ali
Orfali, Raha
Alam, Perwez
Alhowiriny, Tawfeq Abdullah
Al-Yahya, Sami
Majrashi, Najwa Mohammed
Mehmood, Rashad
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Nepeta deflersiana (Lamiaceae) is a well-known medicinal plant that grows in Saudi Arabia.
This plant is used in Saudi and Yemeni folk medicine as an anti-inflammatory, carminative, and antirheumatic agent.
In order to prove its use in folk medicine, four different extracts from the aerial parts of the plant: petroleum ether, chloroform, ethyl acetate, and n-butanol extracts were subjected to biological assays to screen PPARα and PPARϒ agnostic, antioxidant, anti-inflammatory, and cytotoxic activities.
Ethyl acetate and n-butanol extracts of N.
deflersiana NDEE and NDBE, respectively, showed a decrease in oxidative stress and inhibition of both NF-kB and iNOS activities with no cytotoxic effects on four human cancer cell lines.
Both active extracts were standardized using two bioactive metabolites which were isolated from the aerial parts of the same plant [8-epi-7-deoxyloganic acid (compound 1) and Ursolic acid (compound 2)] by developing a validated HPTLC method.
It was found to provide a sharp and compact band of compound 1 at Rf = 0.07 and Rf = 0.57 for compound 2, using chloroform, methanol, and formic acid (8.9:0.8:0.3, v/v/v) as mobile phase at 550 nm.
Compounds 1 and 2 were found in NDEE by 9.59 %, w/w, and 84.63 %, w/w, respectively, and by 11.97 %, w/w, and 21.26 %, w/w, respectively, in NDBE.
American Psychological Association (APA)
Orfali, Raha& Siddiqui, Nasir Ali& Alam, Perwez& Alhowiriny, Tawfeq Abdullah& Al-Taweel, Areej Mohammad& Al-Yahya, Sami…[et al.]. 2018. Biological Evaluation of Different Extracts of Aerial Parts of Nepeta deflersiana and Standardization of Active Extracts Using 8-Epi-7-Deoxyloganic Acid and Ursolic Acid by Validated HPTLC Method. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156732
Modern Language Association (MLA)
Orfali, Raha…[et al.]. Biological Evaluation of Different Extracts of Aerial Parts of Nepeta deflersiana and Standardization of Active Extracts Using 8-Epi-7-Deoxyloganic Acid and Ursolic Acid by Validated HPTLC Method. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1156732
American Medical Association (AMA)
Orfali, Raha& Siddiqui, Nasir Ali& Alam, Perwez& Alhowiriny, Tawfeq Abdullah& Al-Taweel, Areej Mohammad& Al-Yahya, Sami…[et al.]. Biological Evaluation of Different Extracts of Aerial Parts of Nepeta deflersiana and Standardization of Active Extracts Using 8-Epi-7-Deoxyloganic Acid and Ursolic Acid by Validated HPTLC Method. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156732
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156732