Total Flavones of Rhododendron simsii Planch Flower Protect against Cerebral Ischemia-Reperfusion Injury via the Mechanism of Cystathionine-γ-Lyase-Produced H2S
Joint Authors
Fan, Yi-Fei
Chen, Zhi-Wu
Chen, Shuo
Zhang, Jian-Hua
Hu, You-Yang
Hu, Dong-Hua
Gao, Shan-Shan
Wang, Yu-Ling
Jiao, Yi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Total flavones of Rhododendron simsii Planch flower (TFR) have a significant protective effect against cerebral ischemia-reperfusion injury.
However, its mechanism is unclear.
This study investigated the protection of TFR against cerebral ischemia-reperfusion injury via cystathionine-γ-lyase- (CSE-) produced H2S mechanism.
CSE-/- mice and CSE-siRNA-transfected rat were used.
Relaxation of cerebral basilar artery (CBA), H2S, and CSE mRNA were measured.
TFR significantly inhibited cerebral ischemia-reperfusion-induced abnormal neurological symptom and cerebral infarct in the normal rats and the CSE+/+ mice, but not in the CSE-/- mice, and the inhibition was markedly attenuated in CSE-siRNA-transfected rat; TFR elicited a significant vasorelaxation in rat CBA, and the relaxation was markedly attenuated by removal of endothelium or CSE-siRNA transfection or coapplication of NO synthase inhibitor L-NAME and PGI2 synthase inhibitor Indo.
CSE inhibitor PPG drastically inhibited TFR-evoked vasodilatation resistant to L-NAME and Indo in endothelium-intact rat CBA.
TFR significantly increased CSE mRNA expression in rat CBA endothelial cells and H2S production in rat endothelium-intact CBA.
The increase of H2S production resistant to L-NAME and Indo was abolished by PPG.
Our data indicate that TFR has a protective effect against the cerebral ischemia-reperfusion injury via CSE-produced H2S and endothelial NO and/or PGI2 to relax the cerebral artery.
American Psychological Association (APA)
Chen, Shuo& Zhang, Jian-Hua& Hu, You-Yang& Hu, Dong-Hua& Gao, Shan-Shan& Fan, Yi-Fei…[et al.]. 2018. Total Flavones of Rhododendron simsii Planch Flower Protect against Cerebral Ischemia-Reperfusion Injury via the Mechanism of Cystathionine-γ-Lyase-Produced H2S. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156747
Modern Language Association (MLA)
Chen, Shuo…[et al.]. Total Flavones of Rhododendron simsii Planch Flower Protect against Cerebral Ischemia-Reperfusion Injury via the Mechanism of Cystathionine-γ-Lyase-Produced H2S. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1156747
American Medical Association (AMA)
Chen, Shuo& Zhang, Jian-Hua& Hu, You-Yang& Hu, Dong-Hua& Gao, Shan-Shan& Fan, Yi-Fei…[et al.]. Total Flavones of Rhododendron simsii Planch Flower Protect against Cerebral Ischemia-Reperfusion Injury via the Mechanism of Cystathionine-γ-Lyase-Produced H2S. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156747
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156747