Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model
Joint Authors
Zhao, Bin-Bin
Li, Han-Min
Chen, Lin-Lin
Wang, Ping
Long, Qing-hua
Wang, Chao-yang
Xie, Guang-jing
Bo, Wen-ji
Xu, Bo
Li, Ze-fei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Introduction.
To examine the protective effects of Liu Wei Di Huang Wan formula (LWDH) on liver and orbitofrontal cortex (OFC) injuries in monosodium glutamate (MSG) and partial hepatectomy (PH) rat model.
Methods.
Neonatal Wistar rats were given MSG or saline on postnatal days 2, 4, 6, 8, and 10.
The rats were caged into five groups and treated accordingly at six weeks old as follows: Saline group, Saline+PH group, MSG group, MSG+PH group, and LWDH group (MSG+PH+LWDH).
The PH was performed during week 8 by excision of the left and median hepatic lobes (occupying about 68% of whole liver).On day 8 after the PH, the rats were subjected to an inner OFT before being sacrificed.
The liver and OFC were stained using H&E, ORO, or Nissl staining.
The expression of neurotrophic factors (β-NGF, BDNF) was examined in the OFC lysates by ELISA.
Serum levels of cytokines (IL-1β, VEGF) were examined using the Bio-Plex suspension array.
Results.
LWDH increased the total distance traveled by the animals (p<0.05), and LWDH improved the integrity of the Nissl bodies in the OFC (mean area of the Nissl bodies, p<0.01; mean diameter, p<0.05; mean density, p<0.05; and IOD, p<0.01).
There were less white area in the liver (p>0.05) and decreased hepatic steatosis (p<0.01) in LWDH group.
LWDH administration decreased the expression of serum levels of IL-1β (p>0.05), while it increased VEGF (p>0.05) expression.
LWDH administration increased the expression of BDNF (p>0.05) and β-NGF (p>0.05) in the OFC, all as compared to the MSG+PH group.
Conclusion.
LWDH partly protected the animals from depressive-like behaviors in the MSG+PH-induced liver regeneration neonatal rat model.
LWDH alleviated hepatic injury and steatosis and, furthermore, protected the Nissl body integrity and the growth of neurites.
American Psychological Association (APA)
Zhao, Bin-Bin& Long, Qing-hua& Wang, Chao-yang& Chen, Lin-Lin& Xie, Guang-jing& Bo, Wen-ji…[et al.]. 2018. Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156797
Modern Language Association (MLA)
Zhao, Bin-Bin…[et al.]. Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1156797
American Medical Association (AMA)
Zhao, Bin-Bin& Long, Qing-hua& Wang, Chao-yang& Chen, Lin-Lin& Xie, Guang-jing& Bo, Wen-ji…[et al.]. Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156797
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156797