![](/images/graphics-bg.png)
Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum)
Joint Authors
Iqbal, Muhammad Zaffar
Jamil, Shakra
Shahzad, Rahil
Talha, Ghulam Mohyuddin
Sakhawat, Ghazala
Sajid-ur-Rahman, Ghazala
Sultana, Razia
Source
International Journal of Agronomy
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Sugarcane contributes 60–70% of annual sugar production in the world.
Somaclonal variation has potential to enhance genetic variation present within a species.
Present study was done to optimize an in vitro propagation protocol for sugarcane.
The experiments included four varieties, 9 callus induction media, 27 regeneration media, and 9 root induction media under two-factor factorial CRD.
Data were recorded on callus induction, embryogenic callus formation, shoot elongation (cm), root induction, and plant regeneration.
Statistically significant differences existed between genotypes and treatments for callus induction (%), embryogenic callus formation (%), shoot elongation (cm), root induction, and plant regeneration (%).
All parameters showed dependency on genotypes, culture media, and their interaction.
Highest callus induction (95%) embryogenic callus formation (95%) was observed in callus induction media 5.
Highest plantlet regeneration (98.9%) capacity was observed in regeneration media 11 whereas maximum shoot elongation (12.13 cm) and root induction (8.32) were observed in rooting media 4.
G1 showed best response for all traits and vice versa for G4.
Hence it was concluded that G1, callus induction media 5, regeneration media 11, and rooting media 4 are the best conditions for in vitro propagation of sugarcane.
American Psychological Association (APA)
Jamil, Shakra& Shahzad, Rahil& Talha, Ghulam Mohyuddin& Sakhawat, Ghazala& Sajid-ur-Rahman, Ghazala& Sultana, Razia…[et al.]. 2017. Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum). International Journal of Agronomy،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1156881
Modern Language Association (MLA)
Jamil, Shakra…[et al.]. Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum). International Journal of Agronomy No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1156881
American Medical Association (AMA)
Jamil, Shakra& Shahzad, Rahil& Talha, Ghulam Mohyuddin& Sakhawat, Ghazala& Sajid-ur-Rahman, Ghazala& Sultana, Razia…[et al.]. Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum). International Journal of Agronomy. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1156881
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156881