Aster glehni Extract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPARδ-AMPK Pathway
Joint Authors
Seo, Hong Seog
Han, Yoon-Mi
Lee, Yong-Jik
Jang, Yoo-Na
Kim, Hyun-Min
Jin, Changbae
Kim, Hyoung Ja
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Aster glehni (AG) has been used in cooking and as a medicine to treat various diseases for over hundreds of years in Korea.
To speculate the protective effects of AG on skin barrier, we estimated the protein levels of biomarkers related to skin barrier protection in human keratinocytes, HaCaT cells treated with sodium dodecyl sulfate (SDS), or 2,4-dinitrochlorobenzene (DNCB).
The protein levels for keratin, involucrin, defensin, tumor necrosis factor alpha (TNFα), peroxisome proliferator-activated receptor delta (PPARδ), 5′ adenosine monophosphate-activated protein kinase (AMPK), serine palmitoyltransferase long chain base subunit 2 (SPTLC2), and transient receptor potential cation channel subfamily V member 4 (TRPV4) were evaluated using western blotting or immunocytochemistry in HaCaT cells.
AG extract increased the protein levels of PPARδ, phosphorylated AMPK, SPTLC2, keratin, involucrin, and defensin compared to the SDS or DNCB control group.
However, TNFα expression increased by SDS or DNCB was decreased with AG extract.
The order of action of each regulatory biomarker in AG pathway was identified TRPV4→PPARδ→AMPK from antagonist and siRNA treatment studies.
AG can ameliorate the injury of keratinocytes caused by SDS or DNCB through the sequential regulation of TRPV4→PPARδ→AMPK pathway.
American Psychological Association (APA)
Lee, Yong-Jik& Jang, Yoo-Na& Han, Yoon-Mi& Kim, Hyun-Min& Jin, Changbae& Kim, Hyoung Ja…[et al.]. 2018. Aster glehni Extract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPARδ-AMPK Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1156932
Modern Language Association (MLA)
Lee, Yong-Jik…[et al.]. Aster glehni Extract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPARδ-AMPK Pathway. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1156932
American Medical Association (AMA)
Lee, Yong-Jik& Jang, Yoo-Na& Han, Yoon-Mi& Kim, Hyun-Min& Jin, Changbae& Kim, Hyoung Ja…[et al.]. Aster glehni Extract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPARδ-AMPK Pathway. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1156932
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156932