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Aqueous Extract of Glucoraphanin-Rich Broccoli Sprouts Inhibits Formation of Advanced Glycation End Products and Attenuates Inflammatory Reactions in Endothelial Cells
Joint Authors
Sotokawauchi, Ami
Ishibashi, Yuji
Matsui, Takanori
Yamagishi, Sho-ichi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-08
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
We have previously shown that sulforaphane not only inhibits formation of advanced glycation end products (AGEs) but also exerts anti-inflammatory effects on AGE-exposed human umbilical vein endothelial cells (HUVECs) and AGE-injected rat aortae.
Here we examined the effects of aqueous extract of glucoraphanin-rich broccoli sprouts on formation of AGEs and then investigated whether the extract could attenuate inflammatory or oxidative stress reactions in tumor necrosis factor-alpha (TNF-α)- or AGE-exposed HUVECs.
Fresh broccoli sprouts were homogenized in phosphate-buffered saline and filtered through a gauze.
After centrifugation, clear extract was obtained.
AGE formation was measured by enzyme-linked immunosorbent assay.
Gene expression was evaluated by real-time reverse transcription-polymerase chain reaction.
Reactive oxygen species (ROS) generation were measured using a fluorescent dye.
Five percent broccoli sprout extract inhibited the formation of AGEs, reduced basal gene expressions of monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1,) and receptor for AGEs (RAGE), and upregulated endothelial nitric oxide synthase (eNOS) mRNA levels in HUVECs.
TNF-α upregulated MCP-1, ICAM-1, and RAGE mRNA levels in HUVECs, all of which were attenuated by the treatment with 1% broccoli sprout extract.
Pretreatment of 1% broccoli sprout extract prevented the ROS generation in HUVECs evoked by AGEs.
The present study demonstrates that sulforaphane-rich broccoli sprout extract could inhibit the AGE-RAGE axis and exhibit anti-inflammatory actions in HUVECs.
Supplementation of sulforaphane-rich broccoli sprout extract may play a protective role against vascular injury.
American Psychological Association (APA)
Sotokawauchi, Ami& Ishibashi, Yuji& Matsui, Takanori& Yamagishi, Sho-ichi. 2018. Aqueous Extract of Glucoraphanin-Rich Broccoli Sprouts Inhibits Formation of Advanced Glycation End Products and Attenuates Inflammatory Reactions in Endothelial Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1156976
Modern Language Association (MLA)
Sotokawauchi, Ami…[et al.]. Aqueous Extract of Glucoraphanin-Rich Broccoli Sprouts Inhibits Formation of Advanced Glycation End Products and Attenuates Inflammatory Reactions in Endothelial Cells. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1156976
American Medical Association (AMA)
Sotokawauchi, Ami& Ishibashi, Yuji& Matsui, Takanori& Yamagishi, Sho-ichi. Aqueous Extract of Glucoraphanin-Rich Broccoli Sprouts Inhibits Formation of Advanced Glycation End Products and Attenuates Inflammatory Reactions in Endothelial Cells. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1156976
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156976