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Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro
Joint Authors
Zhào, Hóngyi
Liu, Yu
Zeng, Jing
Li, Dandan
Zhang, Weiwei
Huang, Yonghua
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cerebral ischemia/reperfusion (I/R) injury involves complex events of cellular and molecular processes.
Previous studies suggest that a neurovascular unit (NVU) acts as an intricate network to maintain the neuronal homeostatic microenvironment.
The present study established an NVU model for oxygen-glucose deprivation and reoxygenation (OGD/R) damage, trying to target the major components of the NVU using a coculture of rat neurons, astrocytes, and rat brain microvascular endothelial cells (rBMECs) to investigate the therapeutic effects of troxerutin and cerebroprotein hydrolysate injections (TCHis).
The study observed that OGD/R downregulated the expressions of GAP-43, Claudin-5, and AQP-4 obviously detected by Western blotting and immunocytochemical analysis, respectively, while TCHi ameliorated the effect of OGD/R significantly.
Meanwhile, TCHi alleviated the abnormalities of ultrastructure of neurons and rBMECs induced by OGD/R.
Furthermore, both levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) and cell adhesion molecules (VCAM-1 and ICAM-1) detected by ELISA in NVU supernatant were found elevated significantly through OGD/R, but TCHi ameliorated the trend.
In addition, TCHi also mitigated the increase of proapoptotic factors (Bax, p53, and caspase-3) induced by OGD/R in NVU model statistically.
All these findings demonstrated that TCHis played a protective role, which was reflected in anti-inflammation, antiapoptosis, and blood–brain barrier maintenance.
The results of the study concluded that the NVU is an ideal target and TCHi acts as a neuroprotective agent against cerebral I/R injuries.
American Psychological Association (APA)
Zhào, Hóngyi& Liu, Yu& Zeng, Jing& Li, Dandan& Zhang, Weiwei& Huang, Yonghua. 2018. Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156979
Modern Language Association (MLA)
Zhào, Hóngyi…[et al.]. Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1156979
American Medical Association (AMA)
Zhào, Hóngyi& Liu, Yu& Zeng, Jing& Li, Dandan& Zhang, Weiwei& Huang, Yonghua. Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156979
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156979