Apigenin-7-Glucuronide from Urera aurantiaca Inhibits Tumor Necrosis Factor Alpha and Total Nitrite Release in Lipopolysaccharide-Activated Macrophages
Joint Authors
Marrassini, Carla
Cogoi, Laura
Sülsen, Valeria
Anesini, Claudia
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-02
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Urera aurantiaca is an Argentinean medicinal and edible species traditionally used to treat symptoms of inflammation.
The aim of this study was to evaluate the anti-inflammatory activity of a methanol extract and its major compound.
U.
aurantiaca aerial parts were extracted with methanol by maceration.
A phytochemical analysis was performed, and the extract’s major component, apigenin-7-glucuronide (A7G), was identified by spectroscopic and HPLC methods.
The analysis of the inflammatory mediators nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) in lipopolysaccharide- (LPS-) stimulated macrophages were used in the evaluation of the extract and the major compound anti-inflammatory effects.
The extract reduced LPS-augmented NO release from 100 μg/mL (27%), reaching the highest inhibition at 1000 μg/mL (96.3%), while A7G reduced it 30.7% at 1 μg/mL, and its maximum effect was 97.1% at 10 μg/mL.
In the TNF-α model, the extract at 500 and 1000 μg/mL reduced LPS-augmented TNF-α by 13.5% and 93.9%, respectively; meanwhile, A7G reduced it by 26.2% and 83.8% at 5 and 10 μg/mL, respectively.
U.
aurantiaca popular use was validated.
In the present study, for the first time, A7G was isolated from U.
aurantiaca; furthermore, A7G showed anti-inflammatory effect in the macrophage cell line RAW264.7 (ATCC) and seems to be responsible for the extract anti-inflammatory effect.
American Psychological Association (APA)
Marrassini, Carla& Cogoi, Laura& Sülsen, Valeria& Anesini, Claudia. 2020. Apigenin-7-Glucuronide from Urera aurantiaca Inhibits Tumor Necrosis Factor Alpha and Total Nitrite Release in Lipopolysaccharide-Activated Macrophages. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1157014
Modern Language Association (MLA)
Marrassini, Carla…[et al.]. Apigenin-7-Glucuronide from Urera aurantiaca Inhibits Tumor Necrosis Factor Alpha and Total Nitrite Release in Lipopolysaccharide-Activated Macrophages. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1157014
American Medical Association (AMA)
Marrassini, Carla& Cogoi, Laura& Sülsen, Valeria& Anesini, Claudia. Apigenin-7-Glucuronide from Urera aurantiaca Inhibits Tumor Necrosis Factor Alpha and Total Nitrite Release in Lipopolysaccharide-Activated Macrophages. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1157014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157014