Identification of Bioactive Components from Ruellia tuberosa L. on Improving Glucose Uptake in TNF-α-Induced Insulin-Resistant Mouse FL83B Hepatocytes

Joint Authors

Huang, Wen-Chung
Xu, Jian-Hua
Lo, Yangming Martin
Chang, Wen-Chang
Huang, Da-Wei
Wu, James Swi-Bea
Jhang, Yu-Yuan
Ko, Chih-Yuan
Shen, Szu-Chuan

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-01

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Medicine

Abstract EN

Ruellia tuberosa L.

(RTL) has been used as a folk medicine to cure diabetes in Asia.

RTL was previously reported to alleviate hyperglycemia, insulin resistance (IR), abnormal hepatic detoxification, and liver steatosis.

However, the potential bioactive compounds of RTL have still not been identified.

The aim of this study was to investigate the bioactive compounds in RTL ethyl acetate (EA) fractions by using a glucose uptake assay in TNF-α-treated mouse FL83B hepatocytes to discover a mechanism by which to improve IR.

The bioactive compounds were identified by the high-performance liquid chromatography (HPLC) assay.

Using the Sephadex LH20 gel packing chromatography column, the EAF5 fraction was isolated from RTL and significantly increased glucose uptake in TNF-α-treated FL83B cells.

Moreover, the MCI gel packing chromatography column separated EAF5 into five subfractions and had no significant cytotoxic effect in FL83B cells when treated at the concentration of 25 μg/ml.

Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF-α-treated FL83B cells.

The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, p-coumaric acid, and cirsimaritin.

The bioactive compound with the best effect of increasing glucose uptake was p-coumaric acid, but its effect alone was not as good as the combined effect of all three compounds of the EAF5-5 fraction.

Thus, we speculate that the antidiabetic effect of RTL may be the result of multiple active ingredients.

American Psychological Association (APA)

Xu, Jian-Hua& Lo, Yangming Martin& Chang, Wen-Chang& Huang, Da-Wei& Wu, James Swi-Bea& Jhang, Yu-Yuan…[et al.]. 2020. Identification of Bioactive Components from Ruellia tuberosa L. on Improving Glucose Uptake in TNF-α-Induced Insulin-Resistant Mouse FL83B Hepatocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1157019

Modern Language Association (MLA)

Xu, Jian-Hua…[et al.]. Identification of Bioactive Components from Ruellia tuberosa L. on Improving Glucose Uptake in TNF-α-Induced Insulin-Resistant Mouse FL83B Hepatocytes. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1157019

American Medical Association (AMA)

Xu, Jian-Hua& Lo, Yangming Martin& Chang, Wen-Chang& Huang, Da-Wei& Wu, James Swi-Bea& Jhang, Yu-Yuan…[et al.]. Identification of Bioactive Components from Ruellia tuberosa L. on Improving Glucose Uptake in TNF-α-Induced Insulin-Resistant Mouse FL83B Hepatocytes. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1157019

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1157019