Protective Impacts of Moringa oleifera Leaf Extract against Methotrexate-Induced Oxidative Stress and Apoptosis on Mouse Spleen
Joint Authors
Soliman, Mohamed Mohamed
Al-Osaimi, Saad Hommod
HassanMohamed, Essam
Aldhahrani, Adil
Alkhedaide, Adel
Althobaiti, Fayez
Mohamed, Wafaa Abdou
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Objective.
The current study was aimed to examine the possible ameliorative impacts of MO leaf extract (MOLE) against MTX-induced alterations on oxidative stress of mouse spleen and explore the possible molecular mechanism that controls such impacts.
Methods.
Adult male mice were allocated into 4 groups: control, Moringa oleifera leaf extract (MOLE), MTX, and MOLE plus MTX.
Mice received MOLE orally for a week before MTX injection and continued for 12 days.
Serum and spleen were sampled for biochemical and quantitative gene expressions.
Results.
As compared with the MTX-injected group, MOLE effectively reduced the changes in total proteins, spleen MDA, SOD and catalase activities, and changes in serum antioxidants levels.
Moreover, there is downregulation of antioxidant genes (SOD and catalase) and antiapoptotic genes (XIAP and Bcl-xl) along with upregulation in Bax and caspase-3 mRNA (apoptotic genes) in the MTX-injected group.
MTX induced changes in IL-1β, IL-6, TNF-α, and IL-10 expression.
MOLE restored and ameliorated the changes induced in biochemical, antioxidants, apoptosis, and apoptosis associated genes that were induced by MTX intoxication.
Conclusion.
Current findings indicated that pretreatment with MOLE to MTX-intoxicated mice showed the potential usage of MO for oxidative stress and apoptosis treatment.
American Psychological Association (APA)
Soliman, Mohamed Mohamed& Al-Osaimi, Saad Hommod& HassanMohamed, Essam& Aldhahrani, Adil& Alkhedaide, Adel& Althobaiti, Fayez…[et al.]. 2020. Protective Impacts of Moringa oleifera Leaf Extract against Methotrexate-Induced Oxidative Stress and Apoptosis on Mouse Spleen. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1157072
Modern Language Association (MLA)
Soliman, Mohamed Mohamed…[et al.]. Protective Impacts of Moringa oleifera Leaf Extract against Methotrexate-Induced Oxidative Stress and Apoptosis on Mouse Spleen. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1157072
American Medical Association (AMA)
Soliman, Mohamed Mohamed& Al-Osaimi, Saad Hommod& HassanMohamed, Essam& Aldhahrani, Adil& Alkhedaide, Adel& Althobaiti, Fayez…[et al.]. Protective Impacts of Moringa oleifera Leaf Extract against Methotrexate-Induced Oxidative Stress and Apoptosis on Mouse Spleen. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1157072
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157072