Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage
Joint Authors
Tvrdá, Eva
Michalko, Jaroslav
Árvay, Július
Vukovic, Nenad L.
Ivanišová, Eva
Ďuračka, Michal
Matušíková, Ildikó
Kačániová, Miroslava
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-22
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Schisandra chinensis is a woody vine native to China, Korea, and Russia, which has been used as a traditional herbal remedy to treat male infertility.
As very little information is available concerning its effects on ejaculated spermatozoa, the aim of this study was to investigate the chemical, antioxidant, and antibacterial properties of the S.
chinensis berry (Omija) extract followed by an assessment of its in vitro effects on bovine sperm function and oxidative balance.
Phytochemical components of the Omija extract were determined by high performance liquid chromatography.
The content of polyphenols, flavonoids, and carotenoids was assessed by spectrophotometric protocols.
Antioxidant characteristics of the Omija extract were determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and molybdenum-reducing antioxidant power (MRAP) assays.
The disc diffusion method and determination of the minimal inhibitory concentration were applied to study the antibacterial properties of Schisandra.
Thirty semen samples were exposed to different concentrations of Omija (1, 5, 10, 25, 50, and 75 µg/mL) for 0, 2, and 24 h.
Sperm motility, mitochondrial activity, and superoxide and reactive oxygen species production, as well as total antioxidant capacity and oxidative damage to proteins and lipids were determined.
Our data reveal that the Omija extract, particularly at a concentration range within 5–50 µg/mL, exhibited dose-dependent motion-promoting and metabolism-enhancing properties, accompanied by significant antioxidant effects.
We may conclude that the biomolecules present in the Omija extract such as schisandrins and phenolic molecules offer protection to critical sperm structures against oxidative insults and/or possible bacterial contamination, leading to a higher preservation of mammalian sperm viability and functional activity.
American Psychological Association (APA)
Tvrdá, Eva& Michalko, Jaroslav& Árvay, Július& Vukovic, Nenad L.& Ivanišová, Eva& Ďuračka, Michal…[et al.]. 2020. Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1157168
Modern Language Association (MLA)
Tvrdá, Eva…[et al.]. Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1157168
American Medical Association (AMA)
Tvrdá, Eva& Michalko, Jaroslav& Árvay, Július& Vukovic, Nenad L.& Ivanišová, Eva& Ďuračka, Michal…[et al.]. Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1157168
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157168