Gukang Capsule Promotes Fracture Healing by Activating BMPSMAD and Wntβ-Catenin Signaling Pathways
Joint Authors
Wang, Yong-Lin
Wang, Ai-Min
Lan, Yan-Yu
Ma, Xue
Yang, Jian
Liu, Ting
Li, Jing
Tian, Ye
Li, Yongjun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Gukang capsule (GKC) is a traditional Chinese medicine formulation which has been used extensively in the clinical treatment of bone fractures.
However, the mechanisms underlying its effects on fracture healing remain unclear.
Methods.
In this study we used a rabbit radius fracture model, and we measured the serum content of bone alkaline phosphatase (ALP), calcium, and phosphorus and examined pathology of the fracture site as indicators of the fracture healing effects of GKC.
SaOS-2 human osteosarcoma cells were used to measure (i) ALP activity, (ii) ornithine transcarbamylase (OTC), calcium, and mineralization levels, (iii) the expression of osteogenic-related genes, that is, runt-related transcription factor 2 (RUNX2), bone morphogenetic protein 2 (BMP2), collagen I (COL-I), osteopontin (OPN), OTC, and osterix (Osx), and (iv) the expression of key proteins in the Wnt/β-catenin and BMP/SMAD signaling pathways to study the mechanisms by which GKC promotes fracture healing.
Results.
We found that GKC effectively promotes radius fracture healing in rabbits and enhances ALP activity, increases OTC and calcium levels, and stimulates the formation of mineralized nodules in SaOS-2 cells.
Moreover, COL-I, OTC, Osx, BMP2, and OPN expression levels were higher in SaOS-2 cells treated with GKC than control cells.
GKC upregulates glycogen synthase kinase 3β (GSK3β) phosphorylation and Smad1/5 and β-catenin protein levels, thereby activating Wnt/β-catenin and BMP/Smad signaling pathways.
Inhibitors of the Wnt/β-catenin and BMP/Smad signaling pathways (DKK1 and Noggin, respectively) suppress the osteogenic effects of GKC.
Conclusions.
GKC promotes fracture healing by activating the Wnt/β-catenin and BMP/Smad signaling pathways and increasing osteoprotegerin (OPG) secretion by osteoblasts (OBs), which prevents receptor activator of nuclear factor kappa B ligand (RANKL) binding to RANK.
American Psychological Association (APA)
Ma, Xue& Yang, Jian& Liu, Ting& Li, Jing& Lan, Yan-Yu& Wang, Yong-Lin…[et al.]. 2020. Gukang Capsule Promotes Fracture Healing by Activating BMPSMAD and Wntβ-Catenin Signaling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1157183
Modern Language Association (MLA)
Ma, Xue…[et al.]. Gukang Capsule Promotes Fracture Healing by Activating BMPSMAD and Wntβ-Catenin Signaling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1157183
American Medical Association (AMA)
Ma, Xue& Yang, Jian& Liu, Ting& Li, Jing& Lan, Yan-Yu& Wang, Yong-Lin…[et al.]. Gukang Capsule Promotes Fracture Healing by Activating BMPSMAD and Wntβ-Catenin Signaling Pathways. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1157183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157183