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Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK12 and miR-17∼92a Cluster Activation
Joint Authors
Shen, Julian
Wei, Wei
Wang, Xialei
Yang, Jingda
Lu, Lu
Lv, Xinru
Xue, Xiehua
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Context: Alismatis rhizome decoction (AD) exhibits antiatherosclerotic activities.
The activity of AD against vascular smooth muscle cell (VSMC) proliferation remains unclear.
Objective.
The mechanisms and effects of AD on oxidized low-density lipoprotein (ox-LDL)-induced VSMC proliferation were explored.
Materials and methods.
The male SD rats were fed with AD (2.56 g/mL) or 0.9% NaCl by oral gavage 4 mL twice daily for 7 d.
Then, AD-containing serum (ADcs) was collected.
MTS assay was applied to measure the VSMC viability.
The proliferation of VSMCs was detected by 5-bromodeoxyuridine (BrdU) immunocytochemistry.
The microRNA (miRNA) profiling was performed, and the target genes of miRNAs were searched from the TargetScan 7.2 database.
The expressions of matrix metalloproteinases-2/9 (MMP-2/9), cyclin D1/E, cyclin-dependent kinase inhibitor 1B (p27), extracellular regulated protein kinases 1/2 (ERK1/2), and ERK1/2 phosphorylation were examined by western blotting or quantitative reverse transcription PCR.
Results.
The ox-LDL-induced miR-17-92a expression promoted VSMC proliferation.
AD and the ERK1/2 inhibitor U0126 (10 μmol/L) inhibited VSMC proliferation and reduced the overexpression of miR-17∼92a.
AD was found to inhibit phosphorylation of ERK1/2 and reduced the expression of MMP-2/9 in VSMCs.
The expression of cyclin D1/E was suppressed, and p27 was elevated following treatment with AD as well as ERK1/2 inhibitor.
According to the TargetScan 7.2 database, the target genes of miR-17∼92a act on tissue inhibitors of metalloproteinases (TIMPs)-MMPs, p27/21 cyclins, and peroxisome-proliferator-activated receptor α (PPARα) ATP-binding cassette transporter (ABC) A1/G1, which are involved in the process of atherosclerosis.
Conclusions.
AD inhibits ox-LDL-induced VSMC proliferation via inhibiting ERK1/2 and miR-17∼92a activation.
The results provide the multitarget mechanisms for application of AD in the treatment of atherosclerosis.
It would be helpful to the treatment of cardiovascular and cerebral diseases.
American Psychological Association (APA)
Shen, Julian& Wei, Wei& Wang, Xialei& Yang, Jingda& Lu, Lu& Lv, Xinru…[et al.]. 2020. Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK12 and miR-17∼92a Cluster Activation. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1157206
Modern Language Association (MLA)
Shen, Julian…[et al.]. Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK12 and miR-17∼92a Cluster Activation. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1157206
American Medical Association (AMA)
Shen, Julian& Wei, Wei& Wang, Xialei& Yang, Jingda& Lu, Lu& Lv, Xinru…[et al.]. Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK12 and miR-17∼92a Cluster Activation. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1157206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157206