Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages
Joint Authors
Eumkeb, Griangsak
Hengpratom, Tanaporn
Dunkhunthod, Benjawan
Talabnin, Chutima
Murphy, Mark
Sittisart, Patcharawan
Thumanu, Kanjana
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-27
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Oroxylum indicum (L.) Kurz has been used as plant-based food and herbal medicine in many Asian countries.
The aim of the present study was to examine the antioxidant and anti-inflammatory activities of O.
indicum extract (O.
indicum) in RAW264.7 cells activated by LPS plus IFN-γ.
The phytochemical compounds in O.
indicum were identified by GC-MS and LC-MS/MS.
Five flavonoids (luteolin, apigenin, baicalein, oroxylin A, and quercetin) and 27 volatile compounds were found in O.
indicum.
O.
indicum presented antioxidant activities, including reducing ability by FRAP assay and free radical scavenging activity by DPPH assay.
Moreover, O.
indicum also suppressed LPS plus IFN-γ-activated reactive oxygen species generation in RAW264.7 macrophages.
It possessed the potent anti-inflammatory action through suppressing nitric oxide (NO) and IL-6 secretion, possibly due to its ability to scavenge intracellular ROS.
The synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy results showed the alteration of signal intensity and integrated areas relating to lipid and protein of the activated RAW264.7 macrophages compared to unactivated cells.
This is the first report of an application of the SR-FTIR technique to evaluate biomolecular changes in activated RAW264.7 cells.
Our results indicate that O.
indicum may be used as a potential source of nutraceutical for the development of health food supplement or a novel anti-inflammatory herbal medicine.
American Psychological Association (APA)
Dunkhunthod, Benjawan& Talabnin, Chutima& Murphy, Mark& Thumanu, Kanjana& Sittisart, Patcharawan& Hengpratom, Tanaporn…[et al.]. 2020. Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1157243
Modern Language Association (MLA)
Dunkhunthod, Benjawan…[et al.]. Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1157243
American Medical Association (AMA)
Dunkhunthod, Benjawan& Talabnin, Chutima& Murphy, Mark& Thumanu, Kanjana& Sittisart, Patcharawan& Hengpratom, Tanaporn…[et al.]. Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1157243
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157243