Differential Analysis of Serum Principal Components Treated with Compound Sophora Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS)
Joint Authors
Yan, Bin
Sun, Wanjin
Zhou, Conghui
Cai, Quan
He, Hongxia
Duan, Xueyun
Fan, Heng
Zhang, Junjie
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-30
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Objective.
To compare the differences in the serum principal components in ulcerative colitis- (UC-) induced rats, treated with compound Sophora decoction, matrine, oxymatrine monomer mixture, and indirubin monomer, and to provide a modern scientific basis for elucidating the clinical efficacy of compound Sophora decoction for the treatment of UC.
Methods.
The serum samples of rats from each group were obtained after drug administration, and the serum principal components of each group were analyzed by high-resolution mass spectrometry.
Agilent Eclipse XDB C18 chromatographic column (100 mm × 2.1 mm, 3.5 m) was used for separation.
The mobile phase was water (A) and methanol (B) (0.1% formic acid) gradient elution, 0–3 min (B: 20%–40%), 3–10 min (B: 40%–54%), 10–25 min (B: 54%), 25–35 min (B: 54%–70%), 35–45 min (B: 70%–80%), 45–50 min (B: 80%), 50–60 min (B: 80%–100%), 70–72 min (B: 100%–20%), and 72–77 min (B: 20%); flow rate, 300 μL/min; column temperature, 40°C; and injection volume, 10 μL.
ESI source was selected and scanned in the positive and negative ion modes.
The scanning range was 70–1500 m/z; ion-source gas 1 (GS1): 55 psi; ion-source gas 2 (GS2): 60 psi; CUR: 30 psi; ion-source temperature (TEM): 550°C; ion-source voltage (ISVF) : 5500 V/−4500 V; decluster voltage (DP): 100 V; collision energy (CE): 35 V/−35 V; collision energy gain (CES) : 15 V/−15 V; and data acquisition mode: IDA.
After the data from each group were imported into MarkView 1.3, the molecular weights and retention times of different substances were obtained and qualitatively analyzed by ChemSpider and PeakView 2.0.
Results.
In the negative ion mode, 26 differential compounds were identified in the compound Sophora decoction group (FFKST) compared to the model group (M), and 18 differential compounds were identified in the matrine and oxymatrine group (KST) compared to the model group (M).
In the positive ion mode, 11 and 7 differential compounds were identified in the compound Sophora decoction group (FFKST) and the matrine and oxymatrine group (KST) compared to the model group (M), respectively.
The responses of all compounds in each group were compared with each other.
As the different principal component substances in the indirubin group (DYH) displayed little correlation with other groups, the different components in this group were not researched thoroughly.
Conclusion.
By comparing the differences in the serum principal components from each administration group, we found that the FFKST group exhibited enhanced synthesis of the serum principal components; however, the compound doses of matrine and oxymatrine monomers did not exhibit the same changes in the serum principal components of UC-induced rats.
Finally, the traditional Chinese medicine compound is more advantageous than monomers.
American Psychological Association (APA)
Sun, Wanjin& Zhang, Junjie& Zhou, Conghui& Yan, Bin& Cai, Quan& He, Hongxia…[et al.]. 2020. Differential Analysis of Serum Principal Components Treated with Compound Sophora Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS). Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1157286
Modern Language Association (MLA)
Sun, Wanjin…[et al.]. Differential Analysis of Serum Principal Components Treated with Compound Sophora Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS). Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1157286
American Medical Association (AMA)
Sun, Wanjin& Zhang, Junjie& Zhou, Conghui& Yan, Bin& Cai, Quan& He, Hongxia…[et al.]. Differential Analysis of Serum Principal Components Treated with Compound Sophora Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS). Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1157286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157286