![](/images/graphics-bg.png)
Antioxidant Effect of Standardized Extract of Propolis (EPP-AF®) in Healthy Volunteers: A “Before and After” Clinical Study
Joint Authors
Berretta, Andresa Aparecida
Lia, Erica N.
Diniz, Débora P.
Lorencini, Daniela Aparecida
Cintra, Monica A. C. T.
Jordão, Alceu A.
Coelho, Eduardo B.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Propolis is rich in polyphenols, especially flavonoids and phenolic acids, and has significant antioxidant activity, shown mainly in “in vitro” studies.
Objective.
The aim of this study was to evaluate the antioxidant efficacy and safety of a standardized propolis extract in healthy volunteers.
Design.
A two-phase sequential, open-label, nonrandomized, before and after clinical trial.
Methods.
Healthy participants received two EPP-AF® doses (375 and 750 mg/d, P.O, tid) during 7 ± 2 days, starting with the lower doses.
Immediately before starting EPP-AF® administration and at the end of each 7-day dosing schedule, blood and urine samples were collected for quantification of 8-OHDG (8-hydroxydeoxyguanosine) and 8-ISO (8-isoprostanes) in urine and GSH (reduced glutathione), GSSG (oxidized glutathione), SOD (superoxide dismutase), FRAP (Ferric Reducing Antioxidant Power), vitamin E, and MDA (malondialdehyde) in plasma.
Results.
In our study, we had 34 healthy participants (67.7% women, 30 ± 8 years old, 97% white).
The 8-ISO, a biomarker of lipid peroxidation, decreased with both doses of EPP-AF® compared to baseline (8-ISO, 1.1 (0.9–1.3) versus 0.85 (0.75–0.95) and 0.89 (0.74–1.0), ng/mg creatinine, P<0.05, for 375 and 750 mg/d EPP-AF® doses versus baseline, mean and CI 95%, respectively).
8-OHDG, a biomarker of DNA oxidation, was also reduced compared to baseline with 750 mg/d doses (8-OHDG, 15.7 (13.2–18.1) versus 11.6 (10.2–13.0), baseline versus 750 mg/d, respectively, ng/mg creatinine, P<0.05).
Reduction of biomarkers of oxidative stress damage was accompanied by increased plasma SOD activity (68.8 (66.1–73.3) versus 78.2 (72.2–80.5) and 77.7 (74.1–82.6), %inhibition, P<0.0001, 375 and 750 mg/d versus baseline, median and interquartile range 25–75%, respectively) and by increased GSH for 375 mg/d EPP-AF® doses (1.23 (1.06–1.34) versus 1.33 (1.06–1.47), μmol/L, P<0.05).
Conclusion.
EPP-AF® reduced biomarkers of oxidative stress cell damage in healthy humans, with increased antioxidant enzymatic capacity, especially of SOD.
This trial is registered with the Brazilian Registry of Clinical Trials (ReBEC, RBR-9zmfs9).
American Psychological Association (APA)
Diniz, Débora P.& Lorencini, Daniela Aparecida& Berretta, Andresa Aparecida& Cintra, Monica A. C. T.& Lia, Erica N.& Jordão, Alceu A.…[et al.]. 2020. Antioxidant Effect of Standardized Extract of Propolis (EPP-AF®) in Healthy Volunteers: A “Before and After” Clinical Study. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1157301
Modern Language Association (MLA)
Diniz, Débora P.…[et al.]. Antioxidant Effect of Standardized Extract of Propolis (EPP-AF®) in Healthy Volunteers: A “Before and After” Clinical Study. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1157301
American Medical Association (AMA)
Diniz, Débora P.& Lorencini, Daniela Aparecida& Berretta, Andresa Aparecida& Cintra, Monica A. C. T.& Lia, Erica N.& Jordão, Alceu A.…[et al.]. Antioxidant Effect of Standardized Extract of Propolis (EPP-AF®) in Healthy Volunteers: A “Before and After” Clinical Study. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1157301
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157301