Antidiabetic Potential of Prosopis farcta Roots: In Vitro Pancreatic Beta Cell Protection, Enhancement of Glucose Consumption, and Bioassay-Guided Fractionation
Joint Authors
Nabavi, Seyed Mohammad
Habtemariam, Solomon
Shahbazi, Behzad
Feyzmand, Saba
Jafari, Fataneh
Ghiasvand, Nastaran
Bahrami, Gholamreza
Fattahi, Ali
Shokoohinia, Yalda
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
By using the streptozotocin- (STZ-) induced cytotoxicity in β-TC3 cells as an assay model, a bioassay-guided fractionation study was employed to isolate and characterize the potential antidiabetic principles of roots of Prosopis farcta.
A combination of open column chromatography on reverse-phase silica gel using a water-ethanol gradient (10 : 90 to 100 : 0) followed by HPLC-based fractionation led to an active compound that appears to be composed of carbohydrate/sugar.
When cell viability under STZ was reduced to 49.8 ± 4% (mean ± SD), treatment with the active compound at the concentration of 0.5 mg/mL either as a coadministration or a pretreatment improved the viability to 93 ± 1.9% and 91.5 ± 7%, respectively.
The reduction in the mitochondrial membrane potential by STZ (47.34 ± 8.9% of control) was similarly recovered to 84.5 ± 4.3 (coadministration) and 88 ± 5.5% (pretreatment) by the active fraction.
The bioassay-guided fractionation, β-cell protective effect, and increased glucose consumption (up to 1.49-fold increase) in hepatocytes by the extracts and active fraction are also discussed.
American Psychological Association (APA)
Shahbazi, Behzad& Feyzmand, Saba& Jafari, Fataneh& Ghiasvand, Nastaran& Bahrami, Gholamreza& Fattahi, Ali…[et al.]. 2020. Antidiabetic Potential of Prosopis farcta Roots: In Vitro Pancreatic Beta Cell Protection, Enhancement of Glucose Consumption, and Bioassay-Guided Fractionation. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1157384
Modern Language Association (MLA)
Shahbazi, Behzad…[et al.]. Antidiabetic Potential of Prosopis farcta Roots: In Vitro Pancreatic Beta Cell Protection, Enhancement of Glucose Consumption, and Bioassay-Guided Fractionation. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1157384
American Medical Association (AMA)
Shahbazi, Behzad& Feyzmand, Saba& Jafari, Fataneh& Ghiasvand, Nastaran& Bahrami, Gholamreza& Fattahi, Ali…[et al.]. Antidiabetic Potential of Prosopis farcta Roots: In Vitro Pancreatic Beta Cell Protection, Enhancement of Glucose Consumption, and Bioassay-Guided Fractionation. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1157384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157384