New Modified UPLCTandem Mass Spectrometry Method for Determination of Risperidone and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Dose-Dependent Pharmacokinetic Study in Sprague-Dawley Rats
Joint Authors
Ezzeldin, Essam
Tammam, Marwa
Abo Talib, Nisreen F.
Source
International Journal of Analytical Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Sensitive and specific liquid-chromatography tandem mass spectrometry (UPLC-MS/MS) assay has been developed and validated for simultaneous quantification of risperidone (RIS) and its active metabolite 9-hydroxyrisperidone (9-OH-RIS) in rat plasma using olanzapine (OLA) as internal standard (IS).
Pharmacokinetics of risperidone and its active metabolite 9-hydroxyrisperidone was compared across different doses (0.3, 1.0, and 6.0 mg/kg).
Serial blood sample was collected over a time of 48 hours and analyzed for risperidone and its active metabolite 9-hydroxyrisperidone.
The pharmacokinetics parameters including Cmax, tmax, and AUC were determined for risperidone and its active ingredient.
The method was linear in the concentration range of 0.2–500 ng/mL for risperidone and 9-OH-risperidone, with coefficients of determination greater than 0.998 and lower limit of quantitation of 0.2 ng/mL.
Blood levels of risperidone and its active metabolite were roughly dose-proportional.
The method developed herein is simple and rapid and was successfully applied for dose-dependent pharmacokinetic study.
American Psychological Association (APA)
Ezzeldin, Essam& Tammam, Marwa& Abo Talib, Nisreen F.. 2017. New Modified UPLCTandem Mass Spectrometry Method for Determination of Risperidone and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Dose-Dependent Pharmacokinetic Study in Sprague-Dawley Rats. International Journal of Analytical Chemistry،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1157487
Modern Language Association (MLA)
Ezzeldin, Essam…[et al.]. New Modified UPLCTandem Mass Spectrometry Method for Determination of Risperidone and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Dose-Dependent Pharmacokinetic Study in Sprague-Dawley Rats. International Journal of Analytical Chemistry No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1157487
American Medical Association (AMA)
Ezzeldin, Essam& Tammam, Marwa& Abo Talib, Nisreen F.. New Modified UPLCTandem Mass Spectrometry Method for Determination of Risperidone and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Dose-Dependent Pharmacokinetic Study in Sprague-Dawley Rats. International Journal of Analytical Chemistry. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1157487
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157487