Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography
Joint Authors
Jung, Yi-Sook
Lee, Ji Sang
Chung, Yoon-Sok
Chang, Sun Young
Kim, So Hee
Source
International Journal of Analytical Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Pentosidine is an advanced glycation end-product (AGE) and fluorescent cross-link compound.
A simple high-performance liquid chromatographic (HPLC) method was developed for the detection and quantification of pentosidine in human urine and plasma.
The mobile phase used a gradient system to improve separation of pentosidine from endogenous peaks, and chromatograms were monitored by fluorescent detector set at excitation and emission wavelengths of 328 and 378 nm, respectively.
The retention time for pentosidine was 24.3 min and the lower limits of quantification (LLOQ) in human urine and plasma were 1 nM.
The intraday assay precisions (coefficients of variation) were generally low and found to be in the range of 5.19–7.49% and 4.96–8.78% for human urine and plasma, respectively.
The corresponding values of the interday assay precisions were 9.45% and 4.27%.
Accuracies (relative errors) ranged from 87.9% to 115%.
Pentosidine was stable in a range of pH solutions, human urine, and plasma.
In summary, this HPLC method can be applied in future preclinical and clinical evaluation of pentosidine in the diabetic patients.
American Psychological Association (APA)
Lee, Ji Sang& Chung, Yoon-Sok& Chang, Sun Young& Jung, Yi-Sook& Kim, So Hee. 2017. Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography. International Journal of Analytical Chemistry،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1157490
Modern Language Association (MLA)
Lee, Ji Sang…[et al.]. Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography. International Journal of Analytical Chemistry No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1157490
American Medical Association (AMA)
Lee, Ji Sang& Chung, Yoon-Sok& Chang, Sun Young& Jung, Yi-Sook& Kim, So Hee. Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography. International Journal of Analytical Chemistry. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1157490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157490