LncRNA RNCR3 Promotes the Progression of HCC by Activating the AktGSK3β Signaling Pathway
Joint Authors
Zhou, Chunhua
Xie, Qi
Ju, Tongfa
Zhai, Lulu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
To explore the potential biological roles of long noncoding RNA (lncRNA) RNCR3 in human hepatocellular carcinoma (HCC).
Methods.
First, the expression of RNCR3 was detected by qRT-PCR.
Then, in vitro experiments were performed to investigate the effects of RNCR3 on the proliferation, cell cycle, migration, and invasion of HCC cells, while the effects of RNCR3 on HCC tumor growth and metastasis were investigated using in vivo experiments.
Finally, western blot was used to study the activation of the Akt/GSK3β signaling pathway.
Results.
RNCR3 was highly expressed in both HCC tissues and cells, and the expression of RNCR3 was closely related to tumor size, tumor number, TNM stage, and overall survival time.
In vitro, RNCR3 served as an oncogene to promote cell proliferation, migration, and invasion, and in vivo, RNCR3 promoted the growth and metastasis of HCC tumors.
In terms of mechanism, RNCR3 induced the phosphorylation of Akt (thr308 and ser473) and GSK3β (ser9) but decreased the expression of GSK3β, which activated the Akt/GSK3β signaling pathway.
Conclusion.
The high expression of lncRNA RNCR3 in HCC can promote the proliferation, migration, invasion, growth, and metastasis of HCC by activating the NF-κB signaling pathway.
American Psychological Association (APA)
Xie, Qi& Ju, Tongfa& Zhou, Chunhua& Zhai, Lulu. 2020. LncRNA RNCR3 Promotes the Progression of HCC by Activating the AktGSK3β Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157531
Modern Language Association (MLA)
Xie, Qi…[et al.]. LncRNA RNCR3 Promotes the Progression of HCC by Activating the AktGSK3β Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1157531
American Medical Association (AMA)
Xie, Qi& Ju, Tongfa& Zhou, Chunhua& Zhai, Lulu. LncRNA RNCR3 Promotes the Progression of HCC by Activating the AktGSK3β Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157531
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157531