Development of a Rapid and Simple Method to Remove Polyphenols from Plant Extracts
Joint Authors
Soysa, Preethi
Ranatunge, Imali
Adikary, Subshini
Dasanayake, Piumi
Fernando, Chamira Dilanka
Source
International Journal of Analytical Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Polyphenols are secondary metabolites of plants, which are responsible for prevention of many diseases.
Polyvinylpolypyrrolidone (PVPP) has a high affinity towards polyphenols.
This method involves the use of PVPP column to remove polyphenols under centrifugal force.
Standards of gallic acid, epigallocatechin gallate, vanillin, and tea extracts (Camellia sinensis) were used in this study.
PVPP powder was packed in a syringe with different quantities.
The test samples were layered over the PVPP column and subjected to centrifugation.
Supernatant was tested for the total phenol content.
The presence of phenolic compounds and caffeine was screened by HPLC and measuring the absorbance at 280.
The antioxidant capacity of standards and tea extracts was compared with the polyphenol removed fractions using DPPH scavenging assay.
No polyphenols were found in polyphenolic standards or tea extracts after PVPP treatment.
The method described in the present study to remove polyphenols is simple, inexpensive, rapid, and efficient and can be employed to investigate the contribution of polyphenols present in natural products to their biological activity.
American Psychological Association (APA)
Ranatunge, Imali& Adikary, Subshini& Dasanayake, Piumi& Fernando, Chamira Dilanka& Soysa, Preethi. 2017. Development of a Rapid and Simple Method to Remove Polyphenols from Plant Extracts. International Journal of Analytical Chemistry،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1157613
Modern Language Association (MLA)
Ranatunge, Imali…[et al.]. Development of a Rapid and Simple Method to Remove Polyphenols from Plant Extracts. International Journal of Analytical Chemistry No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1157613
American Medical Association (AMA)
Ranatunge, Imali& Adikary, Subshini& Dasanayake, Piumi& Fernando, Chamira Dilanka& Soysa, Preethi. Development of a Rapid and Simple Method to Remove Polyphenols from Plant Extracts. International Journal of Analytical Chemistry. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1157613
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157613