![](/images/graphics-bg.png)
The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells
Joint Authors
Gao, Xiu-Mei
Zhang, Xiting
Li, Lin
Wang, Yi
Mao, Haoping
Chai, Lijuan
Miao, Lin
Wang, Shuang
Zhang, Han
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Shenmai injection (SMI) has been widely used for the treatment of cardiovascular diseases in China.
Cardiovascular disorders are often related to excessive catecholamine (CA) secretion.
Here, we report the effects of SMI on CA secretion and synthesis in cultured bovine adrenal medullary cells.
We found that SMI significantly reduced CA secretion induced by 300 μM acetylcholine (ACh).
Cotreatment with SMI (10 μL/mL) and either of the ACh receptor α-subunit inhibitors, HEX (α3) or DhβE (α4β2), did not produce any further inhibition, indicating that SMI may play a role through α3 and α4β2 channels.
Furthermore, SMI reduced tyrosine hydroxylase (TH) activity induced by ACh by inhibiting the phosphorylation of TH at Ser19 and Ser40.
TH is phosphorylated at Ser19 by Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) and at Ser40 by protein kinase A (PKA).
KN-93 and H89, the antagonists of CaM kinase II and PKA, respectively, inhibited the ACh-induced phosphorylation at Ser19 and Ser40, and the addition of SMI did not augment the inhibitory effect.
Taken together, our results show that SMI likely inhibits CA secretion by blocking TH activity at its Ser19 and Ser40 sites.
American Psychological Association (APA)
Zhang, Xiting& Li, Lin& Wang, Yi& Mao, Haoping& Chai, Lijuan& Miao, Lin…[et al.]. 2020. The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1157665
Modern Language Association (MLA)
Zhang, Xiting…[et al.]. The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1157665
American Medical Association (AMA)
Zhang, Xiting& Li, Lin& Wang, Yi& Mao, Haoping& Chai, Lijuan& Miao, Lin…[et al.]. The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1157665
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157665