Chemical Components and Hepatoprotective Mechanism of Xwak Granule in Mice Treated with Acute Alcohol
Joint Authors
Abdulla, Rahima
Xin, Xuelei
Chen, Li
Liu, Liu
Tursun, Xirali
Aisa, Haji A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Objective.
To evaluate the hepatoprotective mechanism of Xwak granule (Xwak) in treatment of mice with alcoholic liver injury via activating ERK/NF-κB and Nrf/HO-1 signaling pathways.
Methods.
The chemical composition of Xwak was tested by liquid chromatography coupled with mass spectrometry (LC-MS).
Herein, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical tests were performed in vitro.
The hepatoprotective effect of Xwak was assessed at different concentrations (1.5, 3, and 6 g/kg) in a mouse model of alcoholic liver injury.
Results.
Totally, 48 compounds, including 16 flavonoids, 8 tannins, 9 chlorogenic acids, and 15 other compounds, were identified from Xwak.
Xwak showed to have a satisfactory antioxidant activity in vitro.
In a group of Xwak-treated mice, the serum levels of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) were decreased compared with a group of the mouse model of alcoholic liver injury.
In addition, the levels of antioxidant enzymes, such as glutathione peroxidase (GSH-PX), total superoxide dismutase (T-SOD), and catalase (CAT), were noticeably increased and the levels of malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and interleukin-6 (IL-6) were markedly reduced in the liver of mice.
The state of oxidative stress in the mouse model of alcoholic liver injury was improved after treatment with Xwak.
The improvement of inflammation-mediated disruption may conducive to the Xwak activity in the control of liver injury.
The signals of p-ERK1/2, p-NF-κB, COX-2, iNOS, CYP2E1, Nrf, and HO-1 were significantly induced in the liver of mice after treatment with Xwak.
Conclusions.
The abovementioned findings indicated that the hepatoprotective mechanism of Xwak could be achieved by activating ERK/NF-κB and Nrf/HO-1 signaling pathways to alleviate oxidative stress and inflammatory.
American Psychological Association (APA)
Chen, Li& Liu, Liu& Abdulla, Rahima& Tursun, Xirali& Xin, Xuelei& Aisa, Haji A.. 2020. Chemical Components and Hepatoprotective Mechanism of Xwak Granule in Mice Treated with Acute Alcohol. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1157706
Modern Language Association (MLA)
Chen, Li…[et al.]. Chemical Components and Hepatoprotective Mechanism of Xwak Granule in Mice Treated with Acute Alcohol. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1157706
American Medical Association (AMA)
Chen, Li& Liu, Liu& Abdulla, Rahima& Tursun, Xirali& Xin, Xuelei& Aisa, Haji A.. Chemical Components and Hepatoprotective Mechanism of Xwak Granule in Mice Treated with Acute Alcohol. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1157706
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157706