Antioxidant and Anti-Inflammatory Activities of Agrimonia pilosa Ledeb. Extract
Joint Authors
Kim, Choon Young
Yu, Qi-Ming
Kong, Hyun-Joo
Lee, Joo-Yeon
Yang, Kyung-Mi
Seo, Jung-Sook
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The purpose of this study is to investigate the effect of Agrimonia pilosa Ledeb.
extract (APLE) on lipopolysaccharide- (LPS-) induced cell damage in hepatocytes with a focus on antioxidant and anti-inflammatory activities.
Total antioxidant and anti-inflammatory activities of APLE itself were analyzed and phytochemical analysis was performed.
Moreover, inhibitory effects of APLE on LPS-induced oxidative stress and inflammation were assessed in human HepG2 hepatocytes.
APLE was found to exert α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and nitrite scavenging activities and reducing power in a dose-dependent manner.
The total phenolic and flavonoid contents of APLE were 44.30 ± 1.61 mg GAE/g and 29.65 ± 1.81 mg QE/g, respectively.
HPLC analysis revealed that gallic acid is the major phenolic compound in APLE, followed by rutin, genistein, taxifolin, quercetin, luteolin, and apigenin, in descending order.
Treatment of 100 and 200 μg/mL APLE significantly reduced LPS-stimulated intracellular reactive oxygen species production to the basal level without any cytotoxicity.
Oppositely, APLE reversed LPS-suppressed expression of glutathione peroxidase gene and protein.
Consistent with this result, APLE suppressed LPS-triggered expression of proinflammatory cytokine genes in a dose-dependent manner.
These results reinforce the fact that the antioxidant and anti-inflammatory activity of APLE helps protect hepatocytes from LPS.
Thus, APLE may be utilized as a bioactive ingredient in functional foods.
American Psychological Association (APA)
Kim, Choon Young& Yu, Qi-Ming& Kong, Hyun-Joo& Lee, Joo-Yeon& Yang, Kyung-Mi& Seo, Jung-Sook. 2020. Antioxidant and Anti-Inflammatory Activities of Agrimonia pilosa Ledeb. Extract. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157719
Modern Language Association (MLA)
Kim, Choon Young…[et al.]. Antioxidant and Anti-Inflammatory Activities of Agrimonia pilosa Ledeb. Extract. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1157719
American Medical Association (AMA)
Kim, Choon Young& Yu, Qi-Ming& Kong, Hyun-Joo& Lee, Joo-Yeon& Yang, Kyung-Mi& Seo, Jung-Sook. Antioxidant and Anti-Inflammatory Activities of Agrimonia pilosa Ledeb. Extract. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157719
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157719