HPLC-MSMS Analysis of Aconiti Lateralis Radix Praeparata and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach
Joint Authors
Chang, Yanxu
He, Jun
Ma, Wenjuan
Yao, Guangzhe
Jia, Qi
Ouyang, Huizi
Huang, Xuhua
Shen, Jiayuan
Wang, Wei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Red ginseng is often combined with Aconiti Lateralis Radix Praeparata to reduce alkaloids-related toxicity of the latter.
Such herb-pairing also results in better therapeutic effect in heart failure, as compared to the singular use of either herb.
The purpose of this study was to investigate the effect of Aconiti Lateralis Radix Praeparata and its combination with red ginseng on the activities of CYP450 enzymes in rats.
A sensitive and reliable HPLC-MS/MS method was established and validated for the simultaneous determination of eight probe drugs, phenacetin (CYP1A2), tolbutamide (CYP2C9), omeprazole (CYP2C19), dextromethorphan (CYP2D6), dapsone (CYP3A4), 7-hydroxycoumarin (CYP2A6), bupropion (CYP2B6), and amodiaquine (CYP2C8), in rat plasma using diazepam as internal standard (IS).
The chromatographic separation was performed on a Waters XBridge™ C18 column (2.1 mm × 100 mm, 3.5 μm) using a gradient elution with the mobile phase consisting of acetonitrile and water (containing 0.1% formic acid) at a flow rate of 0.3 mL/min.
The method was successfully applied in evaluating the effect of Aconiti Lateralis Radix Praeparata and red ginseng on the activities of CYP450 enzymes.
The pharmacokinetic results of the eight probe drugs suggested that Aconiti Lateralis Radix Praeparata may inhibit the activity of CYP2A6, CYP2C19, CYP2B6, CYP1A2, CYP3A4, and CYP2C9 enzymes in rats.
Comparison between the two groups, Aconiti Lateralis Radix Praeparata combined with red ginseng and Aconiti Lateralis Radix Praeparata, indicated that red ginseng may inhibit the activity of CYP2D6 and CYP2B6 enzymes while inducing the activity of CYP1A2, CYP3A4, and CYP2C9 enzymes.
American Psychological Association (APA)
Ma, Wenjuan& Wang, Wei& Huang, Xuhua& Yao, Guangzhe& Jia, Qi& Shen, Jiayuan…[et al.]. 2020. HPLC-MSMS Analysis of Aconiti Lateralis Radix Praeparata and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1157762
Modern Language Association (MLA)
Ma, Wenjuan…[et al.]. HPLC-MSMS Analysis of Aconiti Lateralis Radix Praeparata and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1157762
American Medical Association (AMA)
Ma, Wenjuan& Wang, Wei& Huang, Xuhua& Yao, Guangzhe& Jia, Qi& Shen, Jiayuan…[et al.]. HPLC-MSMS Analysis of Aconiti Lateralis Radix Praeparata and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1157762
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157762