Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo
Joint Authors
Abdullah, Rasedee
Rahman, Heshu S.
Abdul Samad, Nozlena
Mohan, Syam
Aziz, Sadat
Marif, Hardi
Ford, Dianne
Amin, Kawa
Othman, Hemn Hassan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-19
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
This study investigated the in vivo antileukemic activity of palladium nanoparticles (Pd@W.tea-NPs) mediated by white tea extract in a murine model.
The cell viability effect of Pd@W.tea-NPs, “blank” Pd nanoparticles, and white tea extract alone was determined in murine leukemia WEHI-3B cells and normal mouse fibroblasts (3T3 cells).
Apoptotic and cell cycle arrest effects of Pd@W.tea-NPs in WEHI-3B cells were evaluated.
The effects of Pd@W.tea-NPs administered orally to leukemic mice at 50 and 100 mg/kg daily over 28 days were evaluated.
Pd@W.tea-NPs reduced the viability of WHEI-3B cells with IC50 7.55 μg/ml at 72 h.
Blank Pd nanoparticles and white tea extract alone had smaller effects on WHEI-3B viability and on normal fibroblasts.
Pd@W.tea-NPs increased the proportion of Annexin V-positive WHEI-3B cells and induced G2/M cell cycle arrest.
Leukemic cells in the spleen were reduced by Pd@W.tea-NPs with an increase in Bax/Bcl-2 and cytochrome-C protein and mRNA levels indicating the activation of the mitochondrial apoptotic pathway.
These effects replicated the effects of ATRA and were not observed using blank Pd nanoparticles.
Pd@W.tea-NPs afford therapeutic efficacy against leukemia likely to pivot on activation of the mitochondrial pathway of apoptotic signaling and hence appear attractive potential candidates for development as a novel anticancer agent.
American Psychological Association (APA)
Othman, Hemn Hassan& Rahman, Heshu S.& Mohan, Syam& Aziz, Sadat& Marif, Hardi& Ford, Dianne…[et al.]. 2020. Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1157938
Modern Language Association (MLA)
Othman, Hemn Hassan…[et al.]. Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1157938
American Medical Association (AMA)
Othman, Hemn Hassan& Rahman, Heshu S.& Mohan, Syam& Aziz, Sadat& Marif, Hardi& Ford, Dianne…[et al.]. Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1157938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157938