Si-Wu-Tang Alleviates Nonalcoholic Fatty Liver Disease via Blocking TLR4-JNK and Caspase-8-GSDMD Signaling Pathways
Joint Authors
Zheng, Shuhui
Wang, Fenglin
Zhang, Yaxing
Zhou, Ge
Chen, Zifeng
Guan, Weibing
Zhang, Jiongshan
Bi, Mingmin
You, Xinchao
Liao, Yangjing
Xu, Kangquan
Yang, Hongzhi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Nonalcoholic fatty liver disease (NAFLD) has high global prevalence; however, the treatments of NAFLD are limited due to lack of approved drugs.
Methods.
Mice were randomly assigned into three groups: Control group, NAFLD group, NAFLD plus Si-Wu-Tang group.
A NAFLD mice model was established by feeding with a methionine- and choline-deficient (MCD) diet for four weeks.
Si-Wu-Tang was given orally by gastric gavage at the beginning of 3rd week, and it lasted for two weeks.
The treatment effects of Si-Wu-Tang were confirmed by examining the change of body weight, serum alanine aminotransferase (ALT) and aspartate transaminase (AST) levels, Oil Red O staining, and hematoxylin and eosin (H&E) staining of the liver samples and accompanied by steatosis grade scores.
The expression and activation of the possible signaling proteins involved in the pathogenesis of NAFLD were determined by western blotting.
Results.
Mice fed with four weeks of MCD diet displayed elevated serum levels of ALT and AST, while there was decreased body weight.
The hepatic Oil Red O staining and H&E staining showed severe liver steatosis with high steatosis grade scores.
All these can be improved by treating with Si-Wu-Tang for two weeks.
Mechanistically, the increased hepatic TLR4 expression and its downstream JNK phosphorylation induced by MCD diet were suppressed by Si-Wu-Tang.
Moreover, the upregulations of Caspase-8, gasdermin D (GSDMD), and cleaved-GSDMD in liver mediated by MCD diet were all inhibited by Si-Wu-Tang.
Conclusions.
Treatment with Si-Wu-Tang improves MCD diet-induced NAFLD in part via blocking TLR4-JNK and Caspase-8-GSDMD signaling pathways, suggesting that Si-Wu-Tang has potential for clinical application in treating NAFLD.
American Psychological Association (APA)
Zhang, Yaxing& Zhou, Ge& Chen, Zifeng& Guan, Weibing& Zhang, Jiongshan& Bi, Mingmin…[et al.]. 2020. Si-Wu-Tang Alleviates Nonalcoholic Fatty Liver Disease via Blocking TLR4-JNK and Caspase-8-GSDMD Signaling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1157950
Modern Language Association (MLA)
Zhang, Yaxing…[et al.]. Si-Wu-Tang Alleviates Nonalcoholic Fatty Liver Disease via Blocking TLR4-JNK and Caspase-8-GSDMD Signaling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1157950
American Medical Association (AMA)
Zhang, Yaxing& Zhou, Ge& Chen, Zifeng& Guan, Weibing& Zhang, Jiongshan& Bi, Mingmin…[et al.]. Si-Wu-Tang Alleviates Nonalcoholic Fatty Liver Disease via Blocking TLR4-JNK and Caspase-8-GSDMD Signaling Pathways. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1157950
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157950