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The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells
Joint Authors
Xuan, Zihua
Ding, Jiemin
Liu, Meng li
Wang, Ning
Jia, Xiaoyi
Liu, Min
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aims.
The aim of this study was to evaluate the protective effects of Er Miao San (EMS) and the regulative function of bone marrow-derived dendritic cells (BMDCs) on adjuvant arthritis (AA) in rats.
Methods.
The ethyl acetate part of EMS (3 g/kg, 1.5 g/kg, and 0.75 g/kg) was orally administered from day 15 after immunization to day 29.
The polyarthritis index and paw swelling were measured, the ankle joint pathological changes were observed using hematoxylin-eosin (HE) staining, and the spleen and thymus index were determined.
Moreover, T and B cell proliferation were determined using the CCK-8 assay.
The expression of BMDC surface costimulatory molecules and inflammatory factors were determined using flow cytometry and ELISA kits, respectively.
Results.
Compared with the AA model rats, the ethyl acetate fraction of EMS obviously reduced paw swelling (from 1.0 to 0.7) and the polyarthritis index (from 12 to 9) P<0.01 and improved the severity of histopathology P<0.01.
The treatment using ethyl acetate fraction of EMS significantly reduced the spleen and thymus index P<0.01 and inhibited T and B cell proliferation P<0.01.
Moreover, EMS significantly modulated the expression of surface costimulatory molecules in BMDCs, including CD40, CD80, CD86, and major histocompatibility complex class II (MHC-II) P<0.01.
The results also showed that the ethyl acetate part of EMS significant inhibited the levels of proinflammatory cytokines interleukin- (IL-) 23 tumor necrosis factor- (TNF-) α and inflammatory factor prostaglandin (PG) E2 in the supernatant of BMDCs.
However, the level of anti-inflammatory cytokine IL-10 was significantly increased P<0.01.
Conclusion.
These results suggest that the ethyl acetate part of EMS has better protective effects on AA rats, probably by regulating the function of BMDCs and modulating the balance of cytokines.
American Psychological Association (APA)
Ding, Jiemin& Liu, Min& Xuan, Zihua& Liu, Meng li& Wang, Ning& Jia, Xiaoyi. 2020. The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157965
Modern Language Association (MLA)
Ding, Jiemin…[et al.]. The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1157965
American Medical Association (AMA)
Ding, Jiemin& Liu, Min& Xuan, Zihua& Liu, Meng li& Wang, Ning& Jia, Xiaoyi. The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157965
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157965