Astragaloside IV Enhances Melanogenesis via the AhR-Dependent AKTGSK-3ββ-Catenin Pathway in Normal Human Epidermal Melanocytes
Joint Authors
Liu, Baoyi
Xie, Yongyi
Wu, Zhouwei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Astragalus membranaceus root has been widely used for repigmentation treatment in vitiligo, but its mechanism is poorly understood.
We sought to investigate the effect of astragaloside IV (AS-IV), a main active extract of the Astragalus membranaceus root, on melanin synthesis in normal human epidermal melanocytes (NHEMs) and to elucidate its underlying mechanisms.
Melanin content, tyrosinase activity, qPCR, western blot, and immunofluorescence were employed.
Specific inhibitors and small interfering RNA were used to investigate the possible pathway.
AS-IV stimulated melanin synthesis and upregulated the expression of melanogenesis-related genes in a concentration-dependent manner in NHEMs.
AS-IV could activate the aryl hydrocarbon receptor (AhR), and AS-IV-induced melanogenesis was inhibited in si-AhR-transfected NHEMs.
In addition, we showed that AS-IV enhanced the phosphorylation of AKT and GSK-3β and nuclear translocation of β-catenin.
AS-IV-induced MITF expression upregulation and melanin synthesis were decreased in the presence of β-catenin inhibitor FH353.
Furthermore, AhR antagonist CH223191 inhibited the activation of AKT/GSK-3β/β-catenin signaling, whereas the expression of CYP1A1 (marker of AhR activation) was not affected by the AKT inhibitor in AS-IV-exposed NHEMs.
Our findings show that AS-IV induces melanogenesis through AhR-dependent AKT/GSK-3β/β-catenin pathway activation and could be beneficial in the therapy for depigmented skin disorders.
American Psychological Association (APA)
Liu, Baoyi& Xie, Yongyi& Wu, Zhouwei. 2020. Astragaloside IV Enhances Melanogenesis via the AhR-Dependent AKTGSK-3ββ-Catenin Pathway in Normal Human Epidermal Melanocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1158084
Modern Language Association (MLA)
Liu, Baoyi…[et al.]. Astragaloside IV Enhances Melanogenesis via the AhR-Dependent AKTGSK-3ββ-Catenin Pathway in Normal Human Epidermal Melanocytes. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1158084
American Medical Association (AMA)
Liu, Baoyi& Xie, Yongyi& Wu, Zhouwei. Astragaloside IV Enhances Melanogenesis via the AhR-Dependent AKTGSK-3ββ-Catenin Pathway in Normal Human Epidermal Melanocytes. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1158084
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158084