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Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation
Joint Authors
He, Caigu
Zheng, Xuehua
Lin, Xiuhong
Chen, Xinying
Shen, Chenyi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-14
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Yunvjian (YNJ) is a traditional Chinese medicine formula adopted to prevent and treat diabetes.
Our previous results from animal experiments showed that YNJ decreased blood glucose.
This study aimed to examine the effect of high glucose and high lipid (HG/HL) conditions on the proliferation and apoptosis of INS-1 cells and the possible protective mechanism of YNJ-medicated serum on INS-1 cells exposed to HG/HL conditions.
INS-1 cells were cultured in RPMI 1640 medium after being passaged.
Then, INS-1 cells in the logarithmic growth phase were collected and divided into five groups: control, HG/HL, HG/HL+5% YNJ-medicated serum, HG/HL+10% YNJ-medicated serum, and HG/HL+20% YNJ-medicated serum.
MTT assay and flow cytometry were used to detect proliferation and apoptosis of INS-1 cells, respectively.
Protein profiles of INS-1 cells were analyzed using a tandem mass tag (TMT) label-based quantitative proteomic approach.
Western blotting was performed to verify the proteomic results.
YNJ-medicated serum significantly promoted INS-1 cell proliferation and inhibited apoptosis.
Proteomic results from the INS-1 cells in the control, HG/HL, and HG/HL+10% YNJ-medicated serum groups showed that 7,468 proteins were identified, of which 6,423 proteins were quantified.
Compared with the HG/HL group,430 differential proteins were upregulated, and 671 were downregulated in the HG/HL+10% YNJ-medicated serum group.
Compared with the control group, 711 differential proteins were upregulated and 455 were downregulated in the HG/HL group, whereas 10 differential proteins were upregulated and 9 were downregulated in the HG/HL+10% YNJ-medicated serum group.
Furthermore, several proteins related to autophagy, including ATG3, ATG2B, GABARAP, WIPI2, and p62/SQSTM1, were verified by western blotting, and these results were consistent with the results obtained from the proteomics analysis.
These results confirmed that the autophagy pathway is critical to glucolipotoxicity in INS-1 cells.
YNJ-medicated serum exhibited a protective effect on INS-1 cells cultured under HG/HL conditions by regulating autophagy genes' expression and restoring the autophagic flux.
American Psychological Association (APA)
He, Caigu& Zheng, Xuehua& Lin, Xiuhong& Chen, Xinying& Shen, Chenyi. 2020. Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1158177
Modern Language Association (MLA)
He, Caigu…[et al.]. Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1158177
American Medical Association (AMA)
He, Caigu& Zheng, Xuehua& Lin, Xiuhong& Chen, Xinying& Shen, Chenyi. Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1158177
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158177