Astragaloside IV Reduces Cerebral IschemiaReperfusion-Induced Blood-Brain Barrier Permeability in Rats by Inhibiting ER Stress-Mediated Apoptosis
Joint Authors
Hou, Bonan
Liu, Rui
Wu, You
Huang, Shuiqing
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Previous studies proved that AS-IV could prevent blood-brain barrier (BBB) against an increase in permeability.
However, its underlying molecular mechanism has not been enlightened yet.
The aim of the study is to reveal the potential protective mechanism of astragaloside IV (AS-IV) on the blood-brain barrier after ischemia-reperfusion.
Methods.
In vivo, AS-IV neurological protection was measured by Long’s five-point scale and 2,3,5-triphenyltetrazolium chloride staining.
AS-IV protection for BBB was observed by Evans blue extravasation technique.
Endoplasmic reticulum stress and apoptosis-related protein levels were measured by western blot with AS-IV intervention.
In vitro, cell apoptosis was analyzed by western blot and flow cytometry.Endoplasmic reticulum stress-related protein levels were quantified through western blot.
Results.
AS-IV treatment could decrease the infarct size in rats’ brain and protect the BBB against Evans blue permeating through brain, after ischemia/reperfusion, significantly.
Further, ischemia/reperfusion or oxygen‐glucose deprivation/reperfusion was found to have an increase in endothelial cell apoptosis proteins, such as Bax, Bcl-2, and caspase-3, and endoplasmic reticulum stress-associated proteins, such as phosphorylated PERK and eIF2α, Bip, and CHOP, which were attenuated by AS-IV treatment.
Conclusions.
AS-IV can effectively protect the blood-brain barrier and reduce the area of cerebral infarction via inhibiting endoplasmic reticulum stress-mediated apoptosis in endothelial cells.
American Psychological Association (APA)
Hou, Bonan& Liu, Rui& Wu, You& Huang, Shuiqing. 2020. Astragaloside IV Reduces Cerebral IschemiaReperfusion-Induced Blood-Brain Barrier Permeability in Rats by Inhibiting ER Stress-Mediated Apoptosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1158346
Modern Language Association (MLA)
Hou, Bonan…[et al.]. Astragaloside IV Reduces Cerebral IschemiaReperfusion-Induced Blood-Brain Barrier Permeability in Rats by Inhibiting ER Stress-Mediated Apoptosis. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1158346
American Medical Association (AMA)
Hou, Bonan& Liu, Rui& Wu, You& Huang, Shuiqing. Astragaloside IV Reduces Cerebral IschemiaReperfusion-Induced Blood-Brain Barrier Permeability in Rats by Inhibiting ER Stress-Mediated Apoptosis. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1158346
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158346