Manuka Honey Modulates the Inflammatory Behavior of a dHL-60 Neutrophil Model under the Cytotoxic Limit
Joint Authors
Minden-Birkenmaier, Benjamin A.
Cherukuri, Kasyap
Smith, Richard A.
Radic, Marko Z.
Bowlin, Gary L.
Source
International Journal of Biomaterials
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Recent work has shown that Manuka honey, an increasingly popular wound additive with potent antibacterial properties, also has anti-inflammatory properties.
However, little research has been done examining its effect on neutrophils.
This study investigates the hypothesis that Manuka honey reduces neutrophil superoxide release and chemotaxis and reduces the activation of the inflammatory nuclear factor-κB (NF-κB) signaling pathway under honey’s cytotoxic limit.
A differentiated HL-60 cell line was used as a neutrophil model and cultured in various concentrations of Manuka honey for 3 and 24 hours to measure cytotoxicity via mitochondrial activity and visual trypan-exclusion count.
Cytochrome C and Boyden chamber assays were used to measure the effect of Manuka honey on superoxide release and chemotaxis toward fMLP, respectively.
Additionally, a Western blot for NF-κB inhibitor α (IκBα) was performed to measure Manuka honey’s effect on the NF-κB pathway via IκBα phosphorylation.
The results indicate a cytotoxic limit of 3-5% v/v.
The presence of 1% honey decreased superoxide release at 24 hours.
The 0.5, 1, and 3% honey concentrations reduced chemotaxis and IκBα phosphorylation in a dose-dependent fashion.
These results suggest that Manuka honey significantly reduces neutrophil recruitment and inflammatory behavior in the wound site in a dose-dependent fashion under the cytotoxic limit.
American Psychological Association (APA)
Minden-Birkenmaier, Benjamin A.& Cherukuri, Kasyap& Smith, Richard A.& Radic, Marko Z.& Bowlin, Gary L.. 2019. Manuka Honey Modulates the Inflammatory Behavior of a dHL-60 Neutrophil Model under the Cytotoxic Limit. International Journal of Biomaterials،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1158388
Modern Language Association (MLA)
Minden-Birkenmaier, Benjamin A.…[et al.]. Manuka Honey Modulates the Inflammatory Behavior of a dHL-60 Neutrophil Model under the Cytotoxic Limit. International Journal of Biomaterials No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1158388
American Medical Association (AMA)
Minden-Birkenmaier, Benjamin A.& Cherukuri, Kasyap& Smith, Richard A.& Radic, Marko Z.& Bowlin, Gary L.. Manuka Honey Modulates the Inflammatory Behavior of a dHL-60 Neutrophil Model under the Cytotoxic Limit. International Journal of Biomaterials. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1158388
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158388