Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α

Joint Authors

Hsieh, Shu-Ling
Wang, Jyh-Jye
Su, Kuan-Hua
Kuo, Ying-Lan
Hsieh, Shuchen
Wu, Chih-Chung

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-22

Country of Publication

Egypt

No. of Pages

13

Main Subjects

Medicine

Abstract EN

Gynura bicolor (Roxb.

and Willd.) DC (G.

bicolor) is generally used as a dietary vegetable and traditional herb in Taiwan and the Far East.

G.

bicolor exerts antioxidant and anti-inflammatory effects and regulates blood lipids and cholesterol.

However, the effects of G.

bicolor on endothelial transmigration and atherosclerosis are not clear.

The present study investigated the effects of G.

bicolor on endothelial permeability and transmigration in human endothelial cells.

We prepared G.

bicolor ether extract (GBEE) for use as the experimental material.

Under TNF-α stimulation, HL-60 cell adherence to EA.hy926 cells, the shape of EA.hy926 cells, and the expression of adhesion molecules and transmigration-related regulatory molecules were analysed after pretreatment with GBEE for 24 h.

GBEE inhibited leukocyte adhesion to endothelial cells, reduced intercellular adhesion molecule-1 (ICAM-1) and platelet endothelial cell adhesion molecule-1 (PECAM-1) expressions, and decreased endothelial monolayer permeability.

GBEE also reduced paracellular transmigration by reducing the levels of reactive oxygen species (ROS), Src phosphorylation, and vascular endothelial-cadherin (VE-cadherin) phosphorylation.

GBEE reduced transcellular migration via inhibition of Ras homolog family member A (RhoA) and Rho-associated protein kinase (ROCK) expression and phosphorylation of the ezrin-radixin-moesin (ERM) protein.

Incubation of EA.hy926 cells with GBEE for 8 h and stimulation with TNF-α for 3 h reduced the phosphorylation of the inhibitor of kappa B (IĸB) and DNA-binding activity of nuclear factor-ĸB (NF-ĸB).

These results suggest that GBEE has a protective effect against endothelial dysfunction via suppression of leukocyte-endothelium adhesion and transmigration.

American Psychological Association (APA)

Hsieh, Shu-Ling& Wang, Jyh-Jye& Su, Kuan-Hua& Kuo, Ying-Lan& Hsieh, Shuchen& Wu, Chih-Chung. 2020. Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158474

Modern Language Association (MLA)

Hsieh, Shu-Ling…[et al.]. Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1158474

American Medical Association (AMA)

Hsieh, Shu-Ling& Wang, Jyh-Jye& Su, Kuan-Hua& Kuo, Ying-Lan& Hsieh, Shuchen& Wu, Chih-Chung. Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158474

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1158474