Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α
Joint Authors
Hsieh, Shu-Ling
Wang, Jyh-Jye
Su, Kuan-Hua
Kuo, Ying-Lan
Hsieh, Shuchen
Wu, Chih-Chung
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-22
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Gynura bicolor (Roxb.
and Willd.) DC (G.
bicolor) is generally used as a dietary vegetable and traditional herb in Taiwan and the Far East.
G.
bicolor exerts antioxidant and anti-inflammatory effects and regulates blood lipids and cholesterol.
However, the effects of G.
bicolor on endothelial transmigration and atherosclerosis are not clear.
The present study investigated the effects of G.
bicolor on endothelial permeability and transmigration in human endothelial cells.
We prepared G.
bicolor ether extract (GBEE) for use as the experimental material.
Under TNF-α stimulation, HL-60 cell adherence to EA.hy926 cells, the shape of EA.hy926 cells, and the expression of adhesion molecules and transmigration-related regulatory molecules were analysed after pretreatment with GBEE for 24 h.
GBEE inhibited leukocyte adhesion to endothelial cells, reduced intercellular adhesion molecule-1 (ICAM-1) and platelet endothelial cell adhesion molecule-1 (PECAM-1) expressions, and decreased endothelial monolayer permeability.
GBEE also reduced paracellular transmigration by reducing the levels of reactive oxygen species (ROS), Src phosphorylation, and vascular endothelial-cadherin (VE-cadherin) phosphorylation.
GBEE reduced transcellular migration via inhibition of Ras homolog family member A (RhoA) and Rho-associated protein kinase (ROCK) expression and phosphorylation of the ezrin-radixin-moesin (ERM) protein.
Incubation of EA.hy926 cells with GBEE for 8 h and stimulation with TNF-α for 3 h reduced the phosphorylation of the inhibitor of kappa B (IĸB) and DNA-binding activity of nuclear factor-ĸB (NF-ĸB).
These results suggest that GBEE has a protective effect against endothelial dysfunction via suppression of leukocyte-endothelium adhesion and transmigration.
American Psychological Association (APA)
Hsieh, Shu-Ling& Wang, Jyh-Jye& Su, Kuan-Hua& Kuo, Ying-Lan& Hsieh, Shuchen& Wu, Chih-Chung. 2020. Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158474
Modern Language Association (MLA)
Hsieh, Shu-Ling…[et al.]. Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1158474
American Medical Association (AMA)
Hsieh, Shu-Ling& Wang, Jyh-Jye& Su, Kuan-Hua& Kuo, Ying-Lan& Hsieh, Shuchen& Wu, Chih-Chung. Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158474