Antiangiogenesis Roles of Exosomes with Fei-Liu-Ping Ointment Treatment are Involved in the Lung Carcinoma with the Lewis Xenograft Mouse Model
Joint Authors
Zheng, Qi
Liu, Haolong
Fan, Huiting
Zhang, Ying
Hou, Wei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-16
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Exosomes display efficient biocompatibility and represent valuable vehicles for drug or effective material delivery in a tumour-therapeutic approach.
Following treatment with Fei-Liu-Ping (FLP) ointment, a traditional Chinese herbal formula, which is used for treating lung cancer patients, could inhibit lung carcinoma growth in clinical and animal studies.
In the present study, the values of VEGF and PDGF, which were closely related to angiogenesis, were estimated in serum and carcinoma tissue exosomes to unveil the FLP effects on angiogenesis.
The common inflammatory factors of IL-6, IL-1β, TNF-α, and TGF-β in serum exosomes were also detected with the Lewis xenograft model.
Methods.
Male C57BL/6 mice were randomly divided into four groups, namely, normal, model, cyclophosphamide (CTX), and FLP treatment groups.
Histological structures were observed and imaged by H&E.
CD31 expressions in tumour tissues were detected by immunofluorescence (IF) and western blot (WB).
VEGF, PDGF, and PDGFR levels in exosomes, serum, tumour, and lung tissues were detected by enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and WB, respectively.
IL-6, IL-1β, TNF-α, and TGF-β levels in exosomes were measured by multiplex immunoassay panels.
Results.
The results showed that FLP had tumour growth inhibition rate (39.31%).
CD31 protein expression was obviously decreased in tumour tissues of CTX- and FLP-treated MO mice, compared to that of MO mice (P<0.05 or P<0.001).
VEGF, PDGF, and PDGFR expression levels with FLP treatment were downregulated in exosomes, serum, tumour, and lung tissues compared to model group (P<0.05 or P<0.01).
The expressions of IL-6, IL-1β, and TNF-α were downregulated in exosomes compared to the model group (P<0.05 or P<0.01).
Conclusions.
This study suggested that FLP had the ability of inhibiting tumourigenesis in a Lewis lung xenograft mouse model, whose therapeutic mechanisms might relate with the downregulation of angiogenesis factor and tumour inflammatory cytokines levels.
American Psychological Association (APA)
Zheng, Qi& Liu, Haolong& Fan, Huiting& Zhang, Ying& Hou, Wei. 2020. Antiangiogenesis Roles of Exosomes with Fei-Liu-Ping Ointment Treatment are Involved in the Lung Carcinoma with the Lewis Xenograft Mouse Model. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1158496
Modern Language Association (MLA)
Zheng, Qi…[et al.]. Antiangiogenesis Roles of Exosomes with Fei-Liu-Ping Ointment Treatment are Involved in the Lung Carcinoma with the Lewis Xenograft Mouse Model. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1158496
American Medical Association (AMA)
Zheng, Qi& Liu, Haolong& Fan, Huiting& Zhang, Ying& Hou, Wei. Antiangiogenesis Roles of Exosomes with Fei-Liu-Ping Ointment Treatment are Involved in the Lung Carcinoma with the Lewis Xenograft Mouse Model. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1158496
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158496