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The Isolation of Hypoglycaemic Compounds from Desmodium canum and Their Synergistic Effect on Blood Glucose Levels in Euglycaemic Sprague Dawley Rats
Joint Authors
Lattibeaudiere, Kemmoy
Porter, Roy
Alexander-Lindo, Ruby Lisa
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Desmodium canum (Strong back) commonly consumed as a tea or tonic is believed to possess hypoglycaemic activity.
This paper sets out to isolate potential hypoglycaemic compounds present within the plant and investigate their synergistic effects on blood glucose levels in euglycaemic Sprague Dawley rats.
The milled plant was sequentially extracted using hexane, ethyl acetate and methanol.
The ethyl acetate extract was subjected to column chromatography yielding seven major fractions and were subsequently bioassayed using the Oral Glucose Tolerance Test (OGTT).
Further chromatographic separation and analysis using Gas Chromatography–Mass Spectroscopy and Fourier Transform Infrared Spectroscopy enabled the identification of two hypoglycaemic compounds, oleic acid (OA) and succinic acid (SA).
These were bioassayed individually and as a cocktail to determine the synergistic effects using OGTT.
Intravenous administration of these compounds individually indicated both are very potent in retarding blood glucose levels.
However, the most significant activity was observed on synergistic administration.
The cocktail (1 : 1) displayed significant hypoglycaemic activity throughout the entire study.
It also significantly differed from OA at the 120 min interval (3.43 ± 0.22 mmol/L vs.
4.98 ± 0.19 mmol/L, resp., p=4.29×10−7) and significantly differed from SA at 30 min (3.95 ± 0.43 mmol/L vs.
5.17 ± 0.32 mmol/L, resp., p=0.003), 90 min (4.35 ± 0.36 mmol/L vs.
5.49 ± 0.69 mmol/L, resp., p=0.04), and 120 min intervals (3.43 ± 0.22 mmol/L vs.
4.94 ± 0.31, resp., p=1.54×10−5).
Oral administration of the cocktail showed comparable potency to that of metformin (p>0.05) throughout the OGTT curve.
The synergistic effects of the naturally isolated compounds yielded higher potency levels than individual administration and when administered orally, the hypoglycaemic effect was similar to that of metformin.
This may assist in paving a way to attempt a novel method in approaching antidiabetic therapy.
American Psychological Association (APA)
Lattibeaudiere, Kemmoy& Porter, Roy& Alexander-Lindo, Ruby Lisa. 2020. The Isolation of Hypoglycaemic Compounds from Desmodium canum and Their Synergistic Effect on Blood Glucose Levels in Euglycaemic Sprague Dawley Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1158519
Modern Language Association (MLA)
Lattibeaudiere, Kemmoy…[et al.]. The Isolation of Hypoglycaemic Compounds from Desmodium canum and Their Synergistic Effect on Blood Glucose Levels in Euglycaemic Sprague Dawley Rats. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1158519
American Medical Association (AMA)
Lattibeaudiere, Kemmoy& Porter, Roy& Alexander-Lindo, Ruby Lisa. The Isolation of Hypoglycaemic Compounds from Desmodium canum and Their Synergistic Effect on Blood Glucose Levels in Euglycaemic Sprague Dawley Rats. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1158519
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158519