Antimalarial Activity of Crude Extract and Solvent Fractions of the Leaves of Bersama abyssinica Fresen. (Melianthaceae) against Plasmodium berghei Infection in Swiss Albino Mice
Joint Authors
Melese Birru, Eshetie
Alehegn, Agumas Alemu
Yesuf, Jibril Seid
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-09
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
Treatment of malaria has been compromised by the emergence of drug-resistant parasites.
Consequently, novel agents are urgently needed from different sources including from medicinal plants.
Thus, the current study aimed at evaluating the antimalarial activity of crude extract and solvent fractions of the leaves of Bersama abyssinica (B.
abyssinica) against Plasmodium berghei infection in Swiss Albino mice.
Method.
A 4-day suppressive test was employed to evaluate the antimalarial effect of crude extract and solvent fractions against early infection.
The curative and prophylactic effects of crude extract and fraction with the highest chemosuppression were further tested by Rane’s test and residual infection procedure.
Parasitemia, survival time, packed cell volume (PCV), body weight, and rectal temperature of mice were used as evaluation parameters.
Windows SPSS version 20 was used to analyze the data and analysis of variance (ANOVA) followed by Tukey’s post hoc test was used to compare data between groups.
Results.
The crude extract and aqueous fraction significantly (P<0.05 to 0.001) suppressed parasitemia followed by protection of PCV reduction resulting in prolonging the survival time but failed to protect body weight and rectal temperature reduction in all tested models.
The ethyl acetate and chloroform fractions also showed significant chemosuppression and PCV protection in the 4-day suppressive test.
The crude extract exhibited a chemosuppression of 49.51%, 57.94%, and 44.11% while the aqueous fraction showed suppression of 47.69%, 51.62%, and 37.07% in 4-day suppressive, curative, and prophylactic tests, respectively, at 400 mg/kg.
Conclusion.
The crude extract and fractions showed fairly moderate antimalarial activity, and the finding supports the traditional claims and previous in vitro studies.
Thus, this may call for further studies to isolate chemical entities for additional safety and efficacy tests.
American Psychological Association (APA)
Alehegn, Agumas Alemu& Yesuf, Jibril Seid& Melese Birru, Eshetie. 2020. Antimalarial Activity of Crude Extract and Solvent Fractions of the Leaves of Bersama abyssinica Fresen. (Melianthaceae) against Plasmodium berghei Infection in Swiss Albino Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1158525
Modern Language Association (MLA)
Alehegn, Agumas Alemu…[et al.]. Antimalarial Activity of Crude Extract and Solvent Fractions of the Leaves of Bersama abyssinica Fresen. (Melianthaceae) against Plasmodium berghei Infection in Swiss Albino Mice. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1158525
American Medical Association (AMA)
Alehegn, Agumas Alemu& Yesuf, Jibril Seid& Melese Birru, Eshetie. Antimalarial Activity of Crude Extract and Solvent Fractions of the Leaves of Bersama abyssinica Fresen. (Melianthaceae) against Plasmodium berghei Infection in Swiss Albino Mice. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1158525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158525