Pretreatment Strategies to Improve Crude Glycerol Utilisation and Metabolite Production by Aspergillus terreus
Joint Authors
Abd Rahim, Muhamad Hafiz
Hasan, Hanan
Lim, Elicia Jitming
Samrani, Phebe K.
Abbas, Ali
Source
International Journal of Chemical Engineering
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-01
Country of Publication
Egypt
No. of Pages
6
Abstract EN
Crude glycerol (CG) can be used as a substrate for microbial bioconversion.
However, due to presence of many impurities, many microorganisms are unable to utilise this substrate efficiently.
The present study is trying to improve CG using as the feedstock of Aspergillus terreus for the production of lovastatin, (+)-geodin, and sulochrin.
The CG was pretreated chemically (solvents) and physically (activated carbon (AC) and water softener (WS)) to separate most of the impurities from the CG.
For solvent pretreatments, petroleum ether (PE) produced the largest increase of lovastatin (92.8%) when compared to positive control and pure glycerol (PG) and up to 820% when compared to negative control (CG).
In contrast, diethyl ether (DE) produced the largest increase in (+)-geodin at 80.81% (versus CG) and 176.23% (versus PG).
The largest increase in toluene (Tol) was observed in sulochrin production, at 67.22% (versus CG) and 183.85% (versus PG).
For physical pretreatments, the pattern of metabolite production in AC (lovastatin: 20.65 mg/L, (+)-geodin: 7.42 mg/L, sulochrin: 11.74 mg/L) resembled PG (lovastatin: 21.8 mg/L, (+)-geodin: 8.60 mg/L, sulochrin: 8.18 mg/L), while WS (lovastatin: 11.25 mg/L, (+)-geodin: 15.38 mg/L, sulochrin: 16.85 mg/L) resembled CG (lovastatin: 7.1 mg/L, (+)-geodin: 17.10 mg/L, sulochrin: 14.78 mg/L) at day 6 of fermentation.
These results indicate that solvent pretreatments on CG are excellent for metabolites production in A.
terreus, depending on the solvents used.
In contrast, physical pretreatments are only feasible for (+)-geodin and sulochrin production.
Therefore, different strategies can be employed to manipulate the A.
terreus bioconversion using improved CG by using a few simple pretreatment strategies.
American Psychological Association (APA)
Abd Rahim, Muhamad Hafiz& Hasan, Hanan& Lim, Elicia Jitming& Samrani, Phebe K.& Abbas, Ali. 2019. Pretreatment Strategies to Improve Crude Glycerol Utilisation and Metabolite Production by Aspergillus terreus. International Journal of Chemical Engineering،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1158607
Modern Language Association (MLA)
Abd Rahim, Muhamad Hafiz…[et al.]. Pretreatment Strategies to Improve Crude Glycerol Utilisation and Metabolite Production by Aspergillus terreus. International Journal of Chemical Engineering No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1158607
American Medical Association (AMA)
Abd Rahim, Muhamad Hafiz& Hasan, Hanan& Lim, Elicia Jitming& Samrani, Phebe K.& Abbas, Ali. Pretreatment Strategies to Improve Crude Glycerol Utilisation and Metabolite Production by Aspergillus terreus. International Journal of Chemical Engineering. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1158607
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158607