Inhibition of HIV-1 Protease by Carpobrotus edulis (L.)
Joint Authors
Bradley, Graeme
Omoruyi, Beauty E.
Ighodaro, David I.
Afolayan, Anthony Jide
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-08
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Carpobrotus edulis (L.) is a plant commonly found in the Eastern Cape Province of South Africa and is used for the general treatment of infections relating to the human immunodeficiency virus (HIV).
HIV-1 protease plays an important role during HIV replication and maturation to its infectious form, and therefore inhibition of the enzyme is one of the main focus areas in drug development.
The inhibitory effect of a water extract of C.
edulis leaves against HIV-1 protease activity was determined using the SensoLyte® 520 HIV-1 protease assay fluorimetric kit and employing a HiLyte Fluor™488/QXL™520 fluorescence resonance energy transfer (FRET) peptide.
Cytotoxicity of the extract towards HeLa Chang cell lines was determined using an in vitro MTT assay, and the phytochemical profile of the extract was determined with FT-IR and LC-MS.
HIV-1 protease activity was inhibited 83.06% (IC50 1.6 mg/ml) (p<0.0001) by the pepstatin A inhibitor control.
Treatment with all C.
edulis extract concentrations (16, 1.6, 0.16, and 0.016 mg/ml) inhibited HIV-1 protease activity significantly (p<0.0001) in a typical dose response manner.
With regards to cytotoxicity, the negative controls containing untreated HeLa Chang cells exhibited high formazan formation rates in contrast with the positive controls, containing curcumin, which reduced formazan formation significantly (p<0.001), exhibiting cytotoxicity towards the cells.
There was no significant (p>0.05) difference in the formazan formation rates between the negative controls and 1, 0.5, 0.125, 0.065, 0.031, and 0.015 mg/ml plant extract, confirming no toxicity of C.
edulis extracts towards HeLa Chang cells.
Major functional phytochemical compounds identified included alcohols, phenols, alkanes, amines, carboxylic acids, and esters.
LC-ESI-TOF/MS analysis revealed the putative identities of main compounds present in the aqueous leaves extract, including some that contribute to its anti-HIV-1 protease action.
American Psychological Association (APA)
Omoruyi, Beauty E.& Ighodaro, David I.& Afolayan, Anthony Jide& Bradley, Graeme. 2020. Inhibition of HIV-1 Protease by Carpobrotus edulis (L.). Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1158662
Modern Language Association (MLA)
Omoruyi, Beauty E.…[et al.]. Inhibition of HIV-1 Protease by Carpobrotus edulis (L.). Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1158662
American Medical Association (AMA)
Omoruyi, Beauty E.& Ighodaro, David I.& Afolayan, Anthony Jide& Bradley, Graeme. Inhibition of HIV-1 Protease by Carpobrotus edulis (L.). Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1158662
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158662