Xiaoqinglong Decoction Protects the Lungs of AECOPD Mice through the AMPKmTOR Signaling Pathway

Abstract EN

Background/Aim.

Chronic obstructive pulmonary disease (COPD) is one of the most common health problems around the world.

Xiaoqinglong decoction (XQLD) has been clinically reported to improve lung function and shorten cough and sputum in AECOPD patients, but its mechanism is still unclear.

This study aims to investigate the effects of XQLD on inflammation and apoptosis of lung tissues and explore the underlying mechanisms in acute exacerbation in COPD (AECOPD) mice.

Method.

Male C57BL/6J mice were used to establish AECOPD model by cigarette smoke and bacterial exposure.

Mice were randomly divided into normal control (NC), AECOPD, XQLD, Compound C (Com C), Com C + XQLD, and Clarithromycin (CLA) groups.

After treatment, the pulmonary function was evaluated by whole-body plethysmograph.

The lung histopathology was observed by HE staining.

The serum levels of IL-6, TNF-α, and COX-2 were detected by ELISA assay.

The apoptotic index was measured by TUNEL assay, and the protein expressions of Bax, Bcl-2, Caspase-3, GRP78, and CHOP in the lung tissues were measured by western blot assay.

Results.

XQLD treatment can improve pulmonary function (PF), ameliorate lung injury, and suppress inflammation and apoptosis of lung tissues.

In addition, XQLD also markedly attenuated endoplasmic reticulum stress (ERS) and activated AMPK/mTOR pathway in the lung tissues of mice with AECOPD.

However, the AMPK inhibitor Compound C decreased the protective effect of XQLD in AECOPD mice.

Conclusion.

These findings suggested that XQLD has protective effect against inflammation and apoptosis in AECOPD mice by attenuating ER stress via AMPK/mTOR pathway.