Effects of Active Oxygen Toothpaste in Supragingival Biofilm Reduction: A Randomized Controlled Clinical Trial

Abstract EN

Gingivitis is still considered a major risk factor for the occurrence and progression of periodontal disease.

The aim of the present study was to compare the long-term (1, 12, and 18 weeks) antiplaque and antigingivitis efficacies of two commercially available toothpastes, Colgate Total® (CT) and BlueM® (BM), against attached supragingival dental plaque and gingival inflammation in an experimental gingivitis model.

A parallel double-blinded randomized clinical trial including 39 dental students who refrained from all plaque control methods (manual or chemical) for 7 days was conducted.

After the establishment of clinical gingivitis, participants were randomized into two experimental groups (CT and BM).

Plaque index (PI) and gingival index (GI) were then calculated according to Turesky’s modified Quigley and Hein index.

Participants were assessed in four time periods (preclinical trial phase (W −1), gingivitis phase (W0), one week (W1), twelve weeks (W12), and eighteen weeks (W18)).

Participants’ stimulated saliva was collected and cultured (either aerobically or anaerobically, 37°C, 48 hours) in each time period (W −1, W0, W1, W12, and W18) for the count of viable colonies.

Obtained data were analyzed using 2-way ANOVA and Tukey’s test (α = 0.05).

No significant differences were found (p>0.05) between experimental groups at W −1.

Significant differences between groups were observed at W0 (p<0.05) for the parameter time period, but not for the interaction between parameters (time period ∗ toothpastes).

Lower bacterial counts were observed in both groups after one week of toothbrushing; however, no significant differences were found between investigated dentifrices.

Intra- and intergroup comparisons revealed that significant differences were not found (p>0.05) between dentifrices at W1, W12, and W18 for both GI and PI.

The present study demonstrated that toothpastes containing active oxygen and lactoferrin (BM) have comparable antiplaque and antigingivitis efficacies with triclosan-containing toothpastes (CT).