Plasma Sterilization Effectively Reduces Bacterial Contamination in Dental Unit Waterlines
Joint Authors
Noopan, Sarocha
Unchui, Phattranit
Techotinnakorn, Supitcha
Ampornaramveth, Ruchanee Salingcarnboriboon
Source
International Journal of Dentistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Objective.
To investigate the effectiveness of plasma sterilization in reducing bacterial contamination and controlling biofilms in dental unit waterlines.
Materials and Methods.
Ten identical dental chair units (DCUs) were used.
Five DCUs were installed with an automated plasma sterilization system (PSS) and the other five were kept as nontreated controls (CTL).
Water flushed from the airotor line served as the output water of the dental unit waterlines (DUWLs).
Water samples were collected at the beginning and on a weekly basis for 4 months.
Water was analyzed for bacterial contamination (CFU/mL).
Scanning electron microscopy (SEM) was used to investigate the amount of biofilm in the waterlines.
Biofilm viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays.
All statistical analyses were performed using the Mann–Whitney U test.
A value of p<0.05 was considered significant.
Results.
The DCU output water was found to be heavily contaminated with bacteria.
Plasma sterilization effectively reduced bacterial contamination from an average of 212 CFU/mL to 8 CFU/mL.
During the entire period of 4 months, the level remained below 500 CFU/mL, the standard level recommended by the Centers for Disease Control and Prevention (CDC) of the USA.
The reduction in the bacterial count was significant compared with the CTL group (p<0.05).
Plasma sterilization could not eradicate the existing biofilms in the waterlines, and it did reduce biofilm mass and viability.
Moreover, treatment with plasma sterilization did not induce a change in the composition of microorganisms, as analyzed by Gram staining.
Conclusion.
Plasma sterilization, which is part of electrochemically activated water, effectively reduces bacterial contamination and reduces biofilms in dental unit waterlines.
American Psychological Association (APA)
Noopan, Sarocha& Unchui, Phattranit& Techotinnakorn, Supitcha& Ampornaramveth, Ruchanee Salingcarnboriboon. 2019. Plasma Sterilization Effectively Reduces Bacterial Contamination in Dental Unit Waterlines. International Journal of Dentistry،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1159012
Modern Language Association (MLA)
Noopan, Sarocha…[et al.]. Plasma Sterilization Effectively Reduces Bacterial Contamination in Dental Unit Waterlines. International Journal of Dentistry No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1159012
American Medical Association (AMA)
Noopan, Sarocha& Unchui, Phattranit& Techotinnakorn, Supitcha& Ampornaramveth, Ruchanee Salingcarnboriboon. Plasma Sterilization Effectively Reduces Bacterial Contamination in Dental Unit Waterlines. International Journal of Dentistry. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1159012
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159012