Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis

Joint Authors

Pham, Thuy Anh Vu
Tran, Thao Thi Phuong
Luong, Ngan Thi My

Source

International Journal of Dentistry

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-05-13

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Dental

Abstract EN

Aim.

The aim of our study was to evaluate whether there was a difference in antimicrobial effect between the PRP of healthy volunteers and that of patients with chronic periodontitis against P.

gingivalis, which was fresh cultured from subgingival plaque.

Methods.

Subgingival plaque of patients with moderate and severe chronic periodontitis was collected to isolate P.

gingivalis.

The PRP of four individuals with healthy periodontium and four patients with moderate and severe periodontitis were collected with a specific kit using a two-centrifuge procedure, and then, the antibacterial properties against P.

gingivalis were tested, through their minimum inhibitory concentration (MIC), adhesion resistance assay, and biofilm susceptibility assay.

Results.

P.

gingivalis was successfully isolated from the subgingival plaque of the 21st patient.

The round, smooth, and black colony appeared in the agar disk after 7–10 days of incubation under anaerobic conditions.

Bacterial identification was performed by MALDI-TOF and confirmed by PCR.

All PRP samples tested showed the ability to inhibit P.

gingivalis growth.

The MIC value (expressed as fraction of PRP) was 1/2, and PRP prevented P.

gingivalis attachment on the disk surface.

However, PRP did not have a strong effect on the suppression of P.

gingivalis biofilm.

Conclusion.

PRP of individuals with healthy periodontium and chronic periodontitis patients showed antibacterial properties against P.

gingivalis.

This material can become an adjunct to periodontal treatment.

American Psychological Association (APA)

Pham, Thuy Anh Vu& Tran, Thao Thi Phuong& Luong, Ngan Thi My. 2019. Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis. International Journal of Dentistry،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1159057

Modern Language Association (MLA)

Pham, Thuy Anh Vu…[et al.]. Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis. International Journal of Dentistry No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1159057

American Medical Association (AMA)

Pham, Thuy Anh Vu& Tran, Thao Thi Phuong& Luong, Ngan Thi My. Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis. International Journal of Dentistry. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1159057

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1159057