Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro
Joint Authors
Sepp, Krisztián
Molnár, Zsolt
László, Anna M.
Alapi, Tünde
Tóth, László
Serester, Andrea
Valkusz, Zsuzsanna
Gálfi, Márta
Radács, Marianna
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Homeostatic disruptor agents, and endocrine disruptor compounds (EDC) specifically, can originate from agricultural and industrial chemicals.
If they modify the adaptation of living organisms as direct (e.g., by altering hormone regulation, membrane functions) and/or indirect (e.g., cell transformation mechanisms) factors, they are classified as EDC.
We aimed to examine the potential endocrine-disrupting effects of phenylurea herbicides (phenuron, monuron, and diuron) on the oxytocin (OT) and arginine-vasopressin (AVP) release of neurohypophysis cell cultures (NH).
In our experiments, monoamine-activated receptor functions of neurohypophyseal cells were used as a model.
In vitro NH were prepared by enzymatic (trypsin, collagenase) and mechanical dissociation.
In the experimental protocol, the basal levels of OT and AVP were determined as controls.
Later, monoamine (epinephrine, norepinephrine, serotonin, histamine, and dopamine) activation (10−6 M, 30 min) and the effects of phenylurea (10−6 M, 60 min) alone and in combination (monoamines 10−6 M, 30 min + phenylureas 10−6 M, 60 min) with monoamine were studied.
OT and AVP hormone contents in the supernatant media were measured by radioimmunoassay.
The monoamine-activated receptor functions of neurohypophyseal cells were modified by the applied doses of phenuron, monuron, and diuron.
It is concluded that the applied phenylurea herbicides are endocrine disruptor agents, at least in vitro for neurohypophysis function.
American Psychological Association (APA)
Sepp, Krisztián& Molnár, Zsolt& László, Anna M.& Alapi, Tünde& Tóth, László& Serester, Andrea…[et al.]. 2019. Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1159339
Modern Language Association (MLA)
Sepp, Krisztián…[et al.]. Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro. International Journal of Endocrinology No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1159339
American Medical Association (AMA)
Sepp, Krisztián& Molnár, Zsolt& László, Anna M.& Alapi, Tünde& Tóth, László& Serester, Andrea…[et al.]. Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1159339
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159339