Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase ActivityExpression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study
Joint Authors
Gluvic, Zoran
Sudar, Emina
Jovanovic, Aleksandra
Isenovic, Esma R.
Resanovic, Ivana
Zaric, Bozidarka
Milacic, Davorka
Isakovic, Radmilo
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This study aimed at examining the early effects of hyperbaric oxygen therapy (HBOT) on inducible nitric oxide synthase (iNOS) activity/expression in lymphocytes of type 1 diabetes mellitus (T1DM) patients.
A group of 19 patients (mean age: 63 ± 2.1) with T1DM and with the peripheral arterial disease were included in this study.
Patients were exposed to 10 sessions of HBOT in the duration of 1 h to 100% oxygen inhalation at 2.4 ATA.
Blood samples were collected for the plasma C-reactive protein (CRP), plasma free fatty acid (FFA), serum nitrite/nitrate, and serum arginase activity measurements.
Expression of iNOS and phosphorylation of p65 subunit of nuclear factor-κB (NFκB-p65), extracellular-regulated kinases 1/2 (ERK1/2), and protein kinase B (Akt) were examined in lymphocyte lysates by Western blot.
After exposure to HBOT, plasma CRP and FFA were significantly decreased (p<0.001).
Protein expression of iNOS and serum nitrite/nitrate levels were decreased (p<0.01), while serum arginase activity was increased (p<0.05) versus before exposure to HBOT.
Increased phosphorylation of NFκB-p65 at Ser536 (p<0.05) and decreased level of NFκB-p65 protein (p<0.001) in lymphocytes of T1DM patients were observed after HBOT.
Decreased phosphorylation of ERK1/2 (p<0.05) and Akt (p<0.05) was detected after HBOT.
Our results indicate that exposure to HBO decreased iNOS activity/expression via decreasing phosphorylation of ERK1/2 and Akt followed by decreased activity of NFκB.
American Psychological Association (APA)
Resanovic, Ivana& Gluvic, Zoran& Zaric, Bozidarka& Sudar, Emina& Jovanovic, Aleksandra& Milacic, Davorka…[et al.]. 2019. Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase ActivityExpression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1159383
Modern Language Association (MLA)
Resanovic, Ivana…[et al.]. Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase ActivityExpression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study. International Journal of Endocrinology No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1159383
American Medical Association (AMA)
Resanovic, Ivana& Gluvic, Zoran& Zaric, Bozidarka& Sudar, Emina& Jovanovic, Aleksandra& Milacic, Davorka…[et al.]. Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase ActivityExpression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1159383
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159383