miR-98-5p Alleviated Epithelial-to-Mesenchymal Transition and Renal Fibrosis via Targeting Hmga2 in Diabetic Nephropathy
Joint Authors
Zhu, Yingchun
Xu, Jiang
Liang, Wenxing
Li, Ji
Feng, Linhong
Zheng, PengXi
Ji, Tingting
Bai, Shoujun
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Recently, microRNAs have been recognized as crucial regulators of diabetic nephropathy (DN) development.
Epithelial-to-mesenchymal transition (EMT) can play a significant role in tubulointerstitial fibrosis, and it is a hallmark of diabetic nephropathy progression.
Nevertheless, the function of miR-98-5p in the modulation of EMT and renal fibrosis during DN remains barely investigated.
Hence, identifying the mechanisms of miR-98-5p in regulating EMT and fibrosis is of huge significance.
In our present research, decreased miR-98-5p was demonstrated in db/db mice and mice mesangial cells treated with the high dose of glucose.
Meanwhile, activated EMT and increased fibrosis was accompanied with the decrease of miR-98-5p in vitro and in vivo.
Additionally, to further find out the roles of miR-98-5p in DN development, overexpression of miR-98-5p was applied.
Firstly, in vivo investigation exhibited that elevation of miR-98-5p restrained proteinuria, serum creatinine, BUN, the EMT process, and fibrosis.
Furthermore, high glucose was able to promote mice mesangial cell proliferation, EMT process, and induced renal fibrosis, which could be prevented by overexpression of miR-98-5p.
Moreover, high mobility group A (HMGA2) can exhibit an important role in diverse biological processes.
Here, HMGA2 was investigated as a target of miR-98-5p currently.
Luciferase reporter assay was conducted and the correlation of miR-98-5p and HMGA2 was validated.
Moreover, it was displayed that HMGA2 was remarkably elevated in db/db mice and mice mesangial cells.
Furthermore, miR-98-5p strongly depressed HMGA2 protein and mRNA levels in mice mesangial cells.
Overall, these revealed miR-98-5p could suppress the EMT process and renal fibrosis through targeting HMGA2 in DN.
American Psychological Association (APA)
Zhu, Yingchun& Xu, Jiang& Liang, Wenxing& Li, Ji& Feng, Linhong& Zheng, PengXi…[et al.]. 2019. miR-98-5p Alleviated Epithelial-to-Mesenchymal Transition and Renal Fibrosis via Targeting Hmga2 in Diabetic Nephropathy. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1159568
Modern Language Association (MLA)
Zhu, Yingchun…[et al.]. miR-98-5p Alleviated Epithelial-to-Mesenchymal Transition and Renal Fibrosis via Targeting Hmga2 in Diabetic Nephropathy. International Journal of Endocrinology No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1159568
American Medical Association (AMA)
Zhu, Yingchun& Xu, Jiang& Liang, Wenxing& Li, Ji& Feng, Linhong& Zheng, PengXi…[et al.]. miR-98-5p Alleviated Epithelial-to-Mesenchymal Transition and Renal Fibrosis via Targeting Hmga2 in Diabetic Nephropathy. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1159568
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159568