Analysis of Regulatory T Cell Subsets and Their Expression of Helios and PD-1 in Patients with Hashimoto Thyroiditis
Joint Authors
Liu, Xiaoyun
Hu, Yifang
Zhang, Lijuan
Chen, Huanhuan
Zheng, Xuqin
Shi, He
Jiang, Lin
Cui, Dai
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Hashimoto thyroiditis (HT) is an autoimmune disease that presumably arises consequent to loss of immune tolerance to autoantigen in thyroid.
Regulatory T cells (Tregs) are considered to play a vital role in maintaining the immune balance, as they own intensive suppressive function.
This study was undertaken to analyze numbers of Tregs and their expressions of Helios and PD-1 in HT patients.
It also aimed to explore the relationship of these with thyroid function and specific autoantibodies.
Peripheral blood mononuclear cells (PBMCs) were extracted from blood of 20 healthy controls (HC) and 42 HT patients with varying thyroid functions (10 overt hypothyroidism, 12 subclinical hypothyroidism, and 20 euthyroidism).
We performed flow cytometry analysis in PBMCs to detect CD4+CD25+Foxp3+Tregs and their subsets, including CD45RO+Foxp3high activated Treg cells (aTregs), CD45RO-Foxp3low resting Tregs cells (rTregs), and CD45RO+Foxp3low secreting Treg cells (sTregs), as well as the expression of Helios and PD-1 on these cells.
The results showed that the percentage of Tregs, aTregs was significantly lower in HT patients and it showed inverse correlation to thyroid function states, in comparison with these in healthy controls.
In addition, patients with HT showed decreased expression of Helios in aTregs, while having increased expression of PD-1 in Tregs and sTregs.
The levels of Tregs, aTregs, and Helios expressing aTregs were all negatively correlated with antithyroid antibodies.
In conclusion, the deficiency of Tregs frequency and aberrant expressions of Helios and PD-1 may possibly contribute to thyroid immune damage in HT.
American Psychological Association (APA)
Hu, Yifang& Zhang, Lijuan& Chen, Huanhuan& Liu, Xiaoyun& Zheng, Xuqin& Shi, He…[et al.]. 2019. Analysis of Regulatory T Cell Subsets and Their Expression of Helios and PD-1 in Patients with Hashimoto Thyroiditis. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1159597
Modern Language Association (MLA)
Hu, Yifang…[et al.]. Analysis of Regulatory T Cell Subsets and Their Expression of Helios and PD-1 in Patients with Hashimoto Thyroiditis. International Journal of Endocrinology No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1159597
American Medical Association (AMA)
Hu, Yifang& Zhang, Lijuan& Chen, Huanhuan& Liu, Xiaoyun& Zheng, Xuqin& Shi, He…[et al.]. Analysis of Regulatory T Cell Subsets and Their Expression of Helios and PD-1 in Patients with Hashimoto Thyroiditis. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1159597
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159597